Uddin Md Giash, Hossain Mohammad Salim, Rahman Md Atiqur, Uddin A H M Mazbah, Bhuiyan Md Shafiullah
Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh.
Biol Trace Elem Res. 2017 Aug;178(2):189-193. doi: 10.1007/s12011-016-0919-5. Epub 2017 Jan 5.
Chronic liver disease (CLD) is associated with the destruction of liver parenchyma cell. It is the main cause of morbidity and mortality in most of the developed countries. Oxidative stress and altered levels of different trace elements in serum have been documented for different diseases including inflammation and many liver diseases. This study aims to evaluate the serum level of malondialdehyde (MDA), nitric oxide (NO), antioxidant vitamin C, C-reactive protein (CRP), and zinc (Zn) in CLD patients and to establish a correlation among the study parameters with the severity of inflammatory conditions of CLD. In this study, CLD patients and healthy volunteers were recruited. Total cholesterol and triglyceride were determined by colorimeter using enzymatic method. Serum non-enzymatic antioxidant vitamin C, reactive oxygen species nitric oxide (NO), and malondialdehyde (MDA) were determined by UV-spectrophotometric method. Trace element (Zn) levels were determined by graphite furnace atomic absorption spectroscopy. Independent sample t test and Pearson's correlation test were performed for statistical analysis using the statistical software package SPSS, Version 20. Studies showed that the MDA (p < 0.001), NO (p < 0.001), and CRP levels were significantly higher in CLD patients than in control subjects. The antioxidant vitamin C (p < 0.001) and trace element zinc (p < 0.001) were comparatively lower in the CLD patients than in control subjects. Elemental Zn showed an inverse relationship with MDA, NO, and CRP but positively correlated with antioxidant capacity, whereas MDA showed a positive correlation with CRP level. Thus, we conclude that attenuated level of Zn and antioxidant in serum play an important role in the inflammatory status of CLD patients by elevating the concentration of MDA, NO, and CRP.
慢性肝病（CLD）与肝实质细胞的破坏有关。它是大多数发达国家发病和死亡的主要原因。氧化应激以及血清中不同微量元素水平的改变已在包括炎症和许多肝脏疾病在内的不同疾病中得到记录。本研究旨在评估CLD患者血清中丙二醛（MDA）、一氧化氮（NO）、抗氧化维生素C、C反应蛋白（CRP）和锌（Zn）的水平，并确定研究参数与CLD炎症状况严重程度之间的相关性。在本研究中，招募了CLD患者和健康志愿者。总胆固醇和甘油三酯采用酶法通过比色计测定。血清非酶抗氧化维生素C、活性氧一氧化氮（NO）和丙二醛（MDA）采用紫外分光光度法测定。微量元素（Zn）水平采用石墨炉原子吸收光谱法测定。使用统计软件包SPSS 20进行独立样本t检验和Pearson相关检验以进行统计分析。研究表明，CLD患者的MDA（p < 0.001）、NO（p < 0.001）和CRP水平显著高于对照组。CLD患者的抗氧化维生素C（p < 0.001）和微量元素锌（p < 0.001）相对低于对照组。元素Zn与MDA、NO和CRP呈负相关，但与抗氧化能力呈正相关，而MDA与CRP水平呈正相关。因此，我们得出结论，血清中Zn和抗氧化剂水平的降低通过提高MDA、NO和CRP的浓度在CLD患者的炎症状态中起重要作用。
