Karul Aslihan B, Karadag Fisun, Yensel Naciye, Altinisik Mustafa, Altun Cagatay, Cildag Orhan
Department of Biochemistry, Adnan Menderes University School of Medicine, Aydin, Turkey.
Biol Trace Elem Res. 2003 Jul;94(1):41-8. doi: 10.1385/BTER:94:1:41.
We searched for serum concentrations of trace elements and correlated them to malondialdehyde (MDA), which is an indirect marker of oxidative stress, in order to clarify if routine evaluation is necessary in chronic obstructive pulmonary disease (COPD) outpatients. Serum concentrations of copper (Cu), zinc (Zn), and magnesium (Mg) were determined by atomic absorption spectrophotometry and iron (Fe) by a ILLab 1800 autoanalyzer with ILLab test kits. Serum MDA concentrations were detected in terms of TBARS (thiobarbituric acid reactive substances) spectrophotometrically. Serum Cu, Zn, Mg, Fe, and MDA concentrations in patient and control groups were all in the normal reference range. The results respectively were as follows: Cu:123 +/- 29.2 and 122.2 +/- 23.4 microg/dL; Zn: 87.8 +/- 17.8 and 96.9 +/- 12.9 microg/dL; Mg: 2.3 +/- 0,5 and 2.04 +/- 0.28 mg/dL; Fe: 73.8 +/-35.5 and 80.7+/-51.2 microg/dL; MDA: 1.09+/-0.11 and 0.95+/-0.06 nmol/L. MDA was not correlated to Cu, Zn, Mg, or Fe (p>0.05 for all). The serum Zn concentration of COPD group was lower than the control group (p=0.042), whereas the Mg concentration was higher (p=0.021). There was no statistical difference in other study parameters. Oxidative stress was not increased in clinically stable, regularly treated COPD patients. Although there was no deficiency in trace elements (Cu, Fe, Mg, and Zn), serum Zn was close to the lower limit of the reference value. There is no need for routine evaluation of trace elements in clinically stable, regularly treated COPD outpatients.
我们检测了慢性阻塞性肺疾病（COPD）门诊患者的血清微量元素浓度，并将其与氧化应激的间接标志物丙二醛（MDA）进行关联，以明确对COPD门诊患者进行常规评估是否必要。采用原子吸收分光光度法测定血清铜（Cu）、锌（Zn）和镁（Mg）的浓度，使用ILLab 1800自动分析仪及ILLab检测试剂盒测定血清铁（Fe）的浓度。采用分光光度法通过硫代巴比妥酸反应物质（TBARS）检测血清MDA浓度。患者组和对照组的血清Cu、Zn、Mg、Fe和MDA浓度均在正常参考范围内。结果分别如下：Cu：123±29.2和122.2±23.4μg/dL；Zn：87.8±17.8和96.9±12.9μg/dL；Mg：2.3±0.5和2.04±0.28mg/dL；Fe：73.8±35.5和80.7±51.2μg/dL；MDA：1.09±0.11和0.95±0.06nmol/L。MDA与Cu、Zn、Mg或Fe均无相关性（所有p>0.05）。COPD组的血清Zn浓度低于对照组（p=0.042），而Mg浓度高于对照组（p=0.021）。其他研究参数无统计学差异。临床稳定、接受规律治疗的COPD患者氧化应激未增加。虽然微量元素（Cu、Fe、Mg和Zn）无缺乏，但血清Zn接近参考值下限。对于临床稳定、接受规律治疗的COPD门诊患者，无需常规评估微量元素。
