School of Food Science and Engineering, South China University of Technology, South China University of Technology, Guangzhou 510640, China.
Food Funct. 2017 Jan 25;8(1):86-95. doi: 10.1039/c6fo01474g.
The flavonoids (POFs) from the leaves of Platycladus orientalis (L.) Franco were purified using six different macroporous adsorption resins including polar resins NKA-9 and ADS-F8, semi-polar resins ADS-17 and AB-8, and non-polar resins D101 and ADS-5. Among semi-polar resins, AB-8 demonstrated the best adsorption and desorption capacities with an adsorption ratio of 86% and a desorption ratio of 52%. According to the Simultaneous Thermogravimetry-Differential Scanning Calorimetry (STA/TG-DSC) analysis, POFs showed three thermally decomposed temperatures (347.6 °C, 437.5 °C and 494.8 °C). The main flavonoids in POFs were identified as esculin, amentoflavone, glabridin, and afromosin. Meanwhile, POFs in the dosage range of 25 to 400 μg mL showed a significant anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophage cells, which could inhibit the secretion of NO, IL-6, and TNF-α through the inhibition of inflammatory-related gene expressions.
从侧柏（Platycladus orientalis（L.）Franco）叶中分离纯化的类黄酮（POFs）采用 6 种不同的大孔吸附树脂进行分离纯化，包括极性树脂 NKA-9 和 ADS-F8、半极性树脂 ADS-17 和 AB-8 以及非极性树脂 D101 和 ADS-5。在半极性树脂中，AB-8 表现出最佳的吸附和解吸能力，吸附率为 86%，解吸率为 52%。根据同步热重-差示扫描量热分析（STA/TG-DSC）分析，POFs 表现出三个热分解温度（347.6°C、437.5°C 和 494.8°C）。POFs 中的主要类黄酮被鉴定为七叶苷、穗花杉双黄酮、光甘草定和阿福豆素。同时，POFs 在 25 至 400 μg mL 的剂量范围内对脂多糖（LPS）诱导的 RAW 264.7 小鼠巨噬细胞表现出显著的抗炎作用，通过抑制炎症相关基因的表达，抑制 NO、IL-6 和 TNF-α的分泌。
