Gao Li, Gou Na, Amakye William Kwame, Wu Jianlin, Ren Jiaoyan
School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, China.
State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Curr Res Food Sci. 2022 Nov 17;5:2251-2260. doi: 10.1016/j.crfs.2022.11.013. eCollection 2022.
Natural plants are rich sources of various bioactive compounds. Consequently, the efficiently isolation of these bioactive components has always attracted considerable attention. Our work aims to demonstrate a framework for bioactivity guided isolation of potential effective compounds from the complex food materials. We demonstrated its application for isolation of phenolic compounds with anti-proliferative activity against colorectal cancer cells (CRCs) from L. Firstly, phenolic rich fraction was successfully identified as the main effective components that could simultaneously suppress the growth of CRCs and inhibit Wnt signaling. In order to obtain the bioactive phenolic constituents, a detailed study was performed by optimizing the purification conditions. Two phenolic rich fractions (40% and 60% ethanol elution fractions) were then obtained by AB-8 macroporous resins under optimized condition. Finally, the main components (65 compounds) were tentatively identified from the 40% ethanol eluant by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) analysis. Notably, there were five of the phytochemicals (Feruloylagmatine, Haploside C, Sagittatin A, Linderagalactone C and Koparin-2'-methyl ether) which were hitherto unidentified in L. fruit. In conclusion, this study showed that under the principle of bioactivity guided strategy, phenolic constituents with potential anti-CRCs activity were isolated from L.
天然植物是各种生物活性化合物的丰富来源。因此，高效分离这些生物活性成分一直备受关注。我们的工作旨在展示一个从复杂食品原料中进行生物活性导向分离潜在有效化合物的框架。我们展示了其在从L.中分离对结肠癌细胞（CRC）具有抗增殖活性的酚类化合物中的应用。首先，富含酚类的部分被成功鉴定为主要有效成分，其可同时抑制CRC的生长并抑制Wnt信号传导。为了获得生物活性酚类成分，通过优化纯化条件进行了详细研究。然后在优化条件下通过AB - 8大孔树脂获得了两个富含酚类的部分（40％和60％乙醇洗脱部分）。最后，通过超高效液相色谱 - 四极杆飞行时间质谱（UHPLC - Q - TOF/MS）分析从40％乙醇洗脱液中初步鉴定出主要成分（65种化合物）。值得注意的是，其中有五种植物化学物质（阿魏酰鹅掌楸碱、哈普洛苷C、矢车菊素A、乌药内酯C和柯帕因 - 2'-甲醚）在L.果实中迄今尚未被鉴定。总之，本研究表明，在生物活性导向策略的原则下，从L.中分离出了具有潜在抗CRC活性的酚类成分。
