Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, P.R. China.
Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, P.R. China.
J Sep Sci. 2017 Mar;40(6):1234-1243. doi: 10.1002/jssc.201601147. Epub 2017 Feb 22.
The fruits of Lycium ruthenicum Murr. have long been used in folk medicine. Nevertheless, detailed information related to its phenolic composition and its quality control remains scarce. In this study, a simple and reproducible method, based on high-performance liquid chromatography combined with chemometrics, was developed to authenticate 18 samples of L. ruthenicum Murr. collected from different parts of China through fingerprint analysis. The main peaks were identified by quadrupole time-of-flight electrospray ionization mass spectrometry. Four phenolics were quantified, and the most abundant phenolic compound in almost all samples was kukoamine A. Hierarchical cluster analysis and principal component analysis were applied to classify these samples. Also, a total of 26 compounds, which were mainly phenolic compounds and anthocyanins, were identified or tentatively identified based on the available literature and standard references. Among these, 16 were reported for the first time in the extract. The results showed that there was no significant difference between L. ruthenicum fruits from different provinces in terms of chemical composition. Also, the fingerprint together with chemometric analyses and quadrupole time-of-flight electrospray ionization mass spectrometry are promising methods for evaluating the quality consistency, identification, and comprehensive evaluation of L. ruthenicum.
黑果枸杞的果实长期以来一直被用于民间医学。然而，关于其酚类成分及其质量控制的详细信息仍然很少。在这项研究中，开发了一种简单且可重现的方法，基于高效液相色谱法结合化学计量学，通过指纹分析对来自中国不同地区的 18 个黑果枸杞样品进行鉴定。主要峰通过四极杆飞行时间电喷雾电离质谱进行鉴定。对四种酚类物质进行了定量分析，几乎所有样品中最丰富的酚类化合物是黑果枸杞酰胺 A。应用层次聚类分析和主成分分析对这些样品进行分类。此外，基于现有文献和标准参考，共鉴定或初步鉴定了 26 种化合物，主要为酚类化合物和花色苷。其中，有 16 种在提取物中首次报道。结果表明，不同省份的黑果枸杞果实之间在化学成分方面没有显著差异。此外，指纹图谱结合化学计量学分析和四极杆飞行时间电喷雾电离质谱是评估黑果枸杞质量一致性、鉴定和综合评价的有前途的方法。
