Milinčić Danijel D, Kostić Aleksandar Ž, Lević Steva, Gašić Uroš M, Božić Dragana D, Suručić Relja, Ilić Tijana D, Nedović Viktor A, Vidović Bojana B, Pešić Mirjana B
Department of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Department of Plant Physiology, Institute for Biological Research Siniša Stanković-National Institute of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
Foods. 2024 Dec 29;14(1):62. doi: 10.3390/foods14010062.
The current trend in food innovations includes developing products containing plant ingredients or extracts rich in bioactive compounds. This study aimed to prepare and characterize skimmed thermally treated goat's milk powders enriched with lyophilized fruit extracts of Murray (GMLR) and L. (GMLB). Proximate analysis, ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), Fourier transform infrared spectroscopy using attenuated total reflection (FTIR-ATR), and electrophoretic analysis were assessed. Total phenolic content (TPC), total protein content, and antioxidant properties of enriched goat milk powders were determined spectrophotometrically, and prebiotic potential was evaluated by the broth microdilution method. A total of 25 phenolic compounds and 18 phenylamides were detected in the enriched goat milk powders. Electrophoretic analysis showed the absence of proteolysis in the prepared powders. The GMLR showed the highest TPC and displayed a ferric ion-reducing power, probably contributed by anthocyanins and some phenylamides. GMLR and GMLB had higher ABTS radical scavenging activity but lower ferrous ion-chelating capacity than control goat's milk powder. GMLB and GMLR in a dose-dependent manner (0.3-5 mg/mL) showed a growth-promoting effect on probiotic strains. In summary, prepared goji/goat milk powders, primarily GMLR, might be used as prebiotic supplements or functional food additives.
当前食品创新的趋势包括开发含有富含生物活性化合物的植物成分或提取物的产品。本研究旨在制备并表征富含冻干的穆雷氏枸杞(GMLR)和枸杞(GMLB)果实提取物的脱脂热处理山羊奶粉。对其进行了近似分析、超高效液相色谱-四极杆飞行时间质谱联用(UPLC-Q-TOF-MS)、衰减全反射傅里叶变换红外光谱(FTIR-ATR)以及电泳分析。通过分光光度法测定了强化山羊奶粉的总酚含量(TPC)、总蛋白含量和抗氧化性能,并采用肉汤微量稀释法评估了益生元潜力。在强化山羊奶粉中总共检测到25种酚类化合物和18种苯酰胺。电泳分析表明所制备的奶粉中不存在蛋白水解现象。GMLR的TPC最高,并表现出铁离子还原能力,这可能是由花青素和一些苯酰胺所致。与对照山羊奶粉相比,GMLR和GMLB具有更高的ABTS自由基清除活性,但亚铁离子螯合能力较低。GMLB和GMLR以剂量依赖性方式(0.3 - 5 mg/mL)对益生菌菌株表现出促生长作用。总之,所制备的枸杞/山羊奶粉,主要是GMLR,可用作益生元补充剂或功能性食品添加剂。