Sheu Ming-Jen, Chiu Chun-Ching, Yang Deng-Jye, Hsu Tsai-Ching, Tzang Bor-Show
1 Division of Gastroenterology and Hepatology, Chi Mei Medical Center , Tainan, Taiwan .
2 Department of Medicinal Chemistry, Chia Nan University of Pharmacy and Science , Tainan, Taiwan .
J Med Food. 2017 Jan;20(1):56-64. doi: 10.1089/jmf.2016.3817. Epub 2017 Jan 6.
The nonstructural protein NS1 of human parvovirus B19 (B19) is known to exacerbate disease activity in systemic lupus erythematosus (SLE). However, no specific medicine for B19 infection is available. The roots of Gentiana macrophylla Pall. (GM), the traditional Chinese medicine "Qinjiao," have been used for centuries to treat rheumatic disease, including SLE. Herein, we aimed to investigate the effects of GM root extract (100 and 300 mg/kg body weight) on B19-NS1-exacerbated liver injury in NZB/W F1 mice; liver tissues were assessed by hematoxylin-eosin staining and immunoblotting. The GM root extract significantly decreased B19-NS1-exacerbated liver inflammation by suppressing the expressions of hepatic inducible nitric oxide synthase, cyclooxygenase type 2 (COX-2), interleukin (IL)-1β proteins, values of serum asparate transaminase (AST) and alanine transaminase (ALT), and lymphocyte infiltration (P < .05). It also significantly reduced the B19-NS1-exacerbated hepatic matrix metalloproteinase-9 (MMP-9) and urokinase plasminogen activator (uPA) expressions by downregulating tumor necrosis factor (TNF)-α/NF-κB (p65) signaling. These findings suggest a therapeutic potential of GM root extract against B19-NS1-exacerbated liver inflammation in SLE.
已知人细小病毒B19（B19）的非结构蛋白NS1会加剧系统性红斑狼疮（SLE）的疾病活动。然而，目前尚无针对B19感染的特效药物。传统中药“秦艽”，即大叶龙胆（GM）的根，几个世纪以来一直用于治疗包括SLE在内的风湿性疾病。在此，我们旨在研究GM根提取物（100和300毫克/千克体重）对NZB/W F1小鼠中B19-NS1加剧的肝损伤的影响；通过苏木精-伊红染色和免疫印迹对肝脏组织进行评估。GM根提取物通过抑制肝诱导型一氧化氮合酶、环氧合酶2（COX-2）、白细胞介素（IL）-1β蛋白的表达、血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的值以及淋巴细胞浸润，显著降低了B19-NS1加剧的肝脏炎症（P＜0.05）。它还通过下调肿瘤坏死因子（TNF）-α/核因子κB（p65）信号通路，显著降低了B19-NS1加剧的肝基质金属蛋白酶-9（MMP-9）和尿激酶型纤溶酶原激活剂（uPA）的表达。这些发现表明GM根提取物对SLE中B19-NS1加剧的肝脏炎症具有治疗潜力。
