Xiao Chengfeng, Robertson R Meldrum
Department of Biology, Queen's University, Kingston, Ontario, Canada.
PLoS One. 2017 Jan 6;12(1):e0168361. doi: 10.1371/journal.pone.0168361. eCollection 2017.
Increasing evidence indicates that the white (w) gene in Drosophila possesses extra-retinal functions in addition to its classical role in eye pigmentation. We have previously shown that w+ promotes fast and consistent locomotor recovery from anoxia, but how w+ modulates locomotor recovery is largely unknown. Here we show that in the absence of w+, several PDE mutants, especially cyclic guanosine monophosphate (cGMP)-specific PDE mutants, display wildtype-like fast locomotor recovery from anoxia, and that during the night time, locomotor recovery was light-sensitive in white-eyed mutant w1118, and light-insensitive in PDE mutants under w1118 background. Data indicate the involvement of cGMP in the modulation of recovery timing and presumably, light-evoked cGMP fluctuation is associated with light sensitivity of locomotor recovery. This was further supported by the observations that w-RNAi-induced delay of locomotor recovery was completely eliminated by upregulation of cGMP through multiple approaches, including PDE mutation, simultaneous overexpression of an atypical soluble guanylyl cyclase Gyc88E, or sildenafil feeding. Lastly, prolonged sildenafil feeding promoted fast locomotor recovery from anoxia in w1118. Taken together, these data suggest that a White-cGMP interaction modulates the timing of locomotor recovery from anoxia.
越来越多的证据表明，果蝇中的白色（w）基因除了在眼睛色素沉着中具有经典作用外，还具有视网膜外功能。我们之前已经表明，w +能促进从缺氧状态快速且一致地恢复运动能力，但w +如何调节运动恢复在很大程度上尚不清楚。在这里，我们表明，在没有w +的情况下，几个磷酸二酯酶（PDE）突变体，尤其是环鸟苷单磷酸（cGMP）特异性PDE突变体，表现出从缺氧状态野生型样的快速运动恢复，并且在夜间，白眼突变体w1118的运动恢复对光敏感，而在w1118背景下的PDE突变体对光不敏感。数据表明cGMP参与了恢复时间的调节，推测光诱发的cGMP波动与运动恢复的光敏感性有关。这进一步得到以下观察结果的支持：通过多种方法上调cGMP，包括PDE突变、同时过表达非典型可溶性鸟苷酸环化酶Gyc88E或给予西地那非，完全消除了w - RNAi诱导的运动恢复延迟。最后，长期给予西地那非促进了w1118从缺氧状态快速恢复运动能力。综上所述，这些数据表明White - cGMP相互作用调节了从缺氧状态恢复运动的时间。
