Zhang Xuemei, Yang Xiaoyan, Wang Hongbin, Li Qinchun, Wang Haijuan, Li Yanyan
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province 650500, China.
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province 650500, China.
Ecotoxicol Environ Saf. 2017 Apr;138:199-205. doi: 10.1016/j.ecoenv.2016.12.031. Epub 2017 Jan 4.
A pot experiment was conducted to compare the content of endogenous trans-zeatin (Z), plant arsenic (As) uptake and physiological indices in the fronds of As-hyperaccumulator (Pteris cretica var. nervosa) and non-hyperaccumulator (Pteris ensiformis). Furthermore, a stepwise regression method was used to study the relationship among determined indices, and the time-course effect of main indices was also investigated under 100mg/kg As stress with time extension. In the 100-200mg/kg As treatments, plant height showed no significant difference and endogenous Z content significantly increased in P. cretica var. nervosa compared to the control, but a significant decrease of height and endogenous Z was observed in P. ensiformis. The concentrations of As (III) and As (V) increased significantly in the fronds of two plants, but this increase was much higher in P. cretica var. nervosa. Compared to the control, the contents of chlorophyll and soluble protein were significantly increased in P. cretica var. nervosa but decreased in P. ensiformis in the 200mg/kg As treatment, respectively. A significant positive correlation was found between the contents of endogenous Z and total As in P. cretica var. nervosa, but such a correlation was not found in P. ensiformis. Additionally, in the time-course effect experiment, a peak value of each index was appeared in the 43rd day in two plants, except for chlorophyll in P. ensiformis, but this value was significantly higher in P. cretica var. nervosa than that in P. ensiformis. In conclusion, a higher endogenous Z content contributed to As accumulation of P. cretica var. nervosa under As stress.
进行了一项盆栽试验，以比较砷超富集植物（凤尾蕨）和非超富集植物（剑叶凤尾蕨）叶片中内源反式玉米素（Z）的含量、植物对砷（As）的吸收以及生理指标。此外，采用逐步回归法研究测定指标之间的关系，并在100mg/kg砷胁迫下随着时间延长研究主要指标的时间进程效应。在100 - 200mg/kg砷处理下，凤尾蕨的株高与对照相比无显著差异，但其内源Z含量显著增加，而剑叶凤尾蕨的株高和内源Z显著降低。两种植物叶片中As（III）和As（V）的浓度均显著增加，但凤尾蕨的增幅更大。在200mg/kg砷处理下，与对照相比，凤尾蕨的叶绿素和可溶性蛋白含量显著增加，而剑叶凤尾蕨则分别降低。凤尾蕨内源Z含量与总砷含量之间存在显著正相关，而剑叶凤尾蕨未发现这种相关性。此外，在时间进程效应试验中，除剑叶凤尾蕨的叶绿素外，两种植物各指标在第43天均出现峰值，但凤尾蕨的该值显著高于剑叶凤尾蕨。总之，较高的内源Z含量有助于凤尾蕨在砷胁迫下积累砷。
