Li Qinchun, Yang Xiaoyan, Wang Hongbin, Wang Haijuan, He Shujuan
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan, 650500, People's Republic of China.
Environ Sci Pollut Res Int. 2016 Dec;23(23):23422-23435. doi: 10.1007/s11356-016-7544-x. Epub 2016 Sep 9.
A field survey was conducted to evaluate soil metal pollution and endogenous trans-zeatin content in the leaves of plants growing at six sites in a metal-polluted area located in Gejiu, Yunnan, China. Five plant species were collected, and the physicochemical properties and concentrations of five metals in the soil were analyzed. The trans-zeatin content in plant leaves was measured by high-performance liquid chromatography. Based on the Nemerow pollution index, the six sites were classified into four levels of pollution (i.e., low, medium, high, and severely high). The degree of soil metal pollution was cadmium (Cd) > arsenic (As) > lead (Pb) > zinc (Zn) > copper (Cu). The leaf trans-zeatin content in Pteris vittata (an arsenic hyperaccumulator) increased significantly by 98.6 % in soil with a severely high level of pollution compared with soil at a low level of pollution. However, in non-hyperaccumulators Bidens pilosa var. radiata and Ageratina adenophora, a significant decrease in leaf trans-zeatin content of 35.6 and 87.6 %, respectively, was observed. The leaf trans-zeatin content in Artemisia argyi also decreased significantly by 73.6 % in high metal-polluted soil compared with that in medium metal-polluted soil. Furthermore, significant correlations were observed between leaf trans-zeatin content in Pteris vittata and As, Pb, and Cd concentrations in the soil; however, either no correlation or a negative one was observed in the other plant species. Therefore, a high content of trans-zeatin in the leaves of Pteris vittata may play an important role in its normal growth and tolerance to metals.
在中国云南个旧的一个金属污染地区，对六个地点生长的植物叶片中的土壤金属污染和内源反式玉米素含量进行了实地调查。采集了五种植物物种，并分析了土壤的理化性质和五种金属的浓度。通过高效液相色谱法测定植物叶片中的反式玉米素含量。根据内梅罗污染指数，将六个地点分为四个污染等级（即低、中、高和严重高）。土壤金属污染程度为镉（Cd）>砷（As）>铅（Pb）>锌（Zn）>铜（Cu）。与低污染水平的土壤相比，在污染严重高的土壤中，蜈蚣草（一种砷超富集植物）叶片中的反式玉米素含量显著增加了98.6%。然而，在非超富集植物白花鬼针草和紫茎泽兰中，叶片反式玉米素含量分别显著下降了35.6%和87.6%。与中度金属污染土壤相比，在高金属污染土壤中，艾草叶片中的反式玉米素含量也显著下降了73.6%。此外，在蜈蚣草叶片反式玉米素含量与土壤中As、Pb和Cd浓度之间观察到显著相关性；然而，在其他植物物种中，要么没有相关性，要么呈负相关。因此，蜈蚣草叶片中高含量的反式玉米素可能在其正常生长和对金属的耐受性中发挥重要作用。
