Enyu Liu, Na Wang, Chuanzong Zhao, Ben Wang, Xiaojuan Wu, Yan Wang, Zequn Li, Jianguo Hong, Jiayong Wang, Benjia Liang, Cheng Peng, Min Zhu, Zongli Zhang
Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
Department of Dermatology, Shandong Provinical Institute of dermatology and venereology, Jinan 250012, Shandong, P.R. China.
Oncotarget. 2017 Mar 21;8(12):19467-19477. doi: 10.18632/oncotarget.14444.
Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC).
The aim of our present study was to investigate the expression of pStat-3 and integrin αvβ6, two proteins' correlation and their clinical significance in GBC tissues.
The expression of pStat-3 and integrin αvβ6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P=0.008, P=0.000, P=0.000 and P=0.036, P=0.001,P=0.000,respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r =0.349, P <0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin αvβ6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR=1.907, P=0.021 and RR=2.046, P=0.038).
The expression levels of pStat-3 and integrin αvβ6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins' correlation.
pStat-3 and integrin αvβ6 were indicators of tumor's progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients.
磷酸化信号转导子和转录激活子3（pStat-3）与整合素αvβ6在癌症的发生和发展中均发挥着重要作用。然而，它们在胆囊癌（GBC）中的表达相关性及临床意义鲜为人知。
本研究旨在探讨pStat-3和整合素αvβ6在GBC组织中的表达、二者的相关性及其临床意义。
pStat-3和整合素αvβ6的表达均与T分期、淋巴结转移状态、TNM分期显著相关（分别为P = 0.008、P = 0.000、P = 0.000以及P = 0.036、P = 0.001、P = 0.000）。免疫组化（IHC）和蛋白质印迹法显示，它们在GBC组织中的表达高于癌旁组织。两种蛋白之间存在中度正相关（r = 0.349，P <0.001）。Kaplan-Meier生存分析和Cox回归模型显示，pStat-3或整合素αvβ6阳性表达的GBC患者2年生存率显著较低（对数秩检验，P分别为0.002和0.000），且任一标志物均可作为不良独立预后因素（RR = 1.907，P = 0.021；RR = 2.046，P = 0.038）。
采用免疫组化（IHC）方法分析97例GBC癌组织和癌旁组织中pStat-3和整合素αvβ6的表达水平，并通过蛋白质印迹法进一步验证。此外，使用SPSS软件观察它们的临床意义以及两种蛋白的相关性。
pStat-3和整合素αvβ6是GBC患者肿瘤进展和预后不良的指标。对它们的进一步研究可能为GBC患者的防治提供有益的治疗靶点。
