Li Daiqiang, Yang Zhulin, Liu Ziru, Zou Qiong, Yuan Yuan
Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China.
Pathol Oncol Res. 2019 Jan;25(1):157-167. doi: 10.1007/s12253-017-0314-3. Epub 2017 Oct 17.
This study was conducted to investigate the expressions of DDR2 and IFITM1 and their clinical and pathological significances in the rare type squamous cell/adenosquamous carcinomas (SC/ASC) and ordinary adenocarcinomas (AC) of gallbladder cancers. DDR2 and IFITM1 expression was examined in 69 SC/ASCs and 146 ACs using EnVision immunohistochemistry. Results showed that the percentage of positive DDR2 and IFITM1 expression was significantly higher in SC/ASC patients with high TNM stage, lymph node metastasis, invasion, and no resection surgery compared to patients with low TNM stages, no lymph node metastasis, no invasion, and resection surgery (P < 0.05 or P < 0.01). The positive rate of DDR2 was significantly higher in SC/ASC patients with large tumor sizes than patients with small tumor sizes (p < 0.05). The percentage of positive DDR2 and IFITM1 expressions was significantly higher in AC patients with high TNM stages that didn't receive resection surgery compared to patients with low TNM stages that did receive resection surgery (P < 0.05 or P < 0.01). The positive rate of IFITM1 was significantly higher in AC patients with lymph node metastasis and invasion than in patients without metastasis and invasion (p < 0.05). Positive DDR2 and IFITM1 expression was closely associated with a decreased overall survival in SC/ASC and AC patients (P < 0.05 or P < 0.01). AUC analysis showed that DDR2 and IFITM1 was sensitive and specific for the diagnosis of SC/ASC (AUC = 0.740 and AUC =0.733, respectively) and AC (AUC = 0.710 and AUC =0.741, respectively). In conclusion, positive DDR2 and IFITM1 expression is a marker for the clinical severity, poor prognosis, and diagnosis of gallbladder SC/ASC and AC.
本研究旨在探讨盘状结构域受体2（DDR2）和干扰素诱导跨膜蛋白1（IFITM1）在胆囊癌的罕见类型鳞状细胞/腺鳞癌（SC/ASC）及普通腺癌（AC）中的表达情况及其临床和病理意义。采用EnVision免疫组织化学法检测69例SC/ASC和146例AC中DDR2和IFITM1的表达。结果显示，与TNM分期低、无淋巴结转移、无浸润且接受手术切除的患者相比，TNM分期高、有淋巴结转移、有浸润且未接受手术切除的SC/ASC患者中DDR2和IFITM1阳性表达百分比显著更高（P<0.05或P<0.01）。肿瘤体积大的SC/ASC患者中DDR2阳性率显著高于肿瘤体积小的患者（P<0.05）。与TNM分期低且接受手术切除的患者相比，TNM分期高且未接受手术切除的AC患者中DDR2和IFITM1阳性表达百分比显著更高（P<0.05或P<0.01）。有淋巴结转移和浸润的AC患者中IFITM1阳性率显著高于无转移和浸润的患者（P<0.05）。DDR2和IFITM1阳性表达与SC/ASC和AC患者总生存期降低密切相关（P<0.05或P<0.01）。受试者工作特征曲线下面积（AUC）分析显示，DDR2和IFITM1对SC/ASC（AUC分别为0.740和0.733）及AC（AUC分别为0.710和0.741）的诊断具有敏感性和特异性。总之，DDR2和IFITM1阳性表达是胆囊SC/ASC和AC临床严重程度、预后不良及诊断的标志物。
