Dai Lei, Wang Yaozong, Chen Liangliang, Zheng Jueru, Li Jianjun, Wu Xianjiang
Department of Thyroid Surgery, Ningbo NO.2 Hospital, NO.41 Xibei Street, Ningbo City, 315000, Zhejiang Province, China.
World J Surg Oncol. 2017 Jan 7;15(1):11. doi: 10.1186/s12957-016-1086-z.
Many studies have reported several transcriptionally deregulated microRNAs (miRNAs) in papillary thyroid cancer (PTC) tissue in comparison with benign thyroid nodules and normal thyroid tissues. However, the correlation between miRNA expressions and PTC recurrence still remains unclear.
The PTC patients who scheduled to undergo total thyroidectomy by the same surgical team in Ningbo NO.2 Hospital from March 1998 to March 2008 were enrolled in this study. The clinical and pathological characteristics of each patient were recorded in detail. The selected miRNA expressions were detected using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Potential predictive factors for cancer recurrence were evaluated by univariate and multivariate Cox proportional hazard analysis.
A total of 78 patients were enrolled with 49 females at a mean age of 45.8 years. Enrolled patients were divided into two groups: nonrecurrent group (n = 54) and recurrent group (n = 24). The results from the univariate Cox proportional hazard analysis revealed that primary tumor size, TNM stage, extrathyroid extension, miR-221, and miR-222 expressions were significantly associated with PTC recurrence (P < 0.05). The tissue expression of miR-221 was the only independent risk factor for PTC recurrence (HR 1.41; 95%CI 1.14-1.95, P = 0.007) by multiple Cox proportional hazard analysis.
This study identified the potential role of miR-221 as a prognostic biomarker for the recurrence in PTC.
许多研究报告称，与良性甲状腺结节和正常甲状腺组织相比，乳头状甲状腺癌（PTC）组织中有几种转录失调的微小RNA（miRNA）。然而，miRNA表达与PTC复发之间的相关性仍不清楚。
本研究纳入了1998年3月至2008年3月在宁波市第二医院由同一手术团队计划进行全甲状腺切除术的PTC患者。详细记录每位患者的临床和病理特征。使用定量逆转录聚合酶链反应（qRT-PCR）检测所选miRNA的表达。通过单因素和多因素Cox比例风险分析评估癌症复发的潜在预测因素。
共纳入78例患者，其中49例女性，平均年龄45.8岁。纳入的患者分为两组：无复发组（n = 54）和复发组（n = 24）。单因素Cox比例风险分析结果显示，原发肿瘤大小、TNM分期、甲状腺外侵犯、miR-221和miR-222表达与PTC复发显著相关（P < 0.05）。多因素Cox比例风险分析显示，miR-221的组织表达是PTC复发的唯一独立危险因素（HR 1.41；95%CI 1.14 - 1.95，P = 0.007）。
本研究确定了miR-221作为PTC复发预后生物标志物的潜在作用。
