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用于研究同源重组的单链DNA帘

Single-Stranded DNA Curtains for Studying Homologous Recombination.

作者信息

Ma C J, Steinfeld J B, Greene E C

机构信息

Columbia University, New York, NY, United States.

Columbia University, New York, NY, United States.

出版信息

Methods Enzymol. 2017;582:193-219. doi: 10.1016/bs.mie.2016.08.005. Epub 2016 Oct 22.

DOI:10.1016/bs.mie.2016.08.005
PMID:28062035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7229809/
Abstract

Homologous recombination is an important pathway involved in the repair of double-stranded DNA breaks. Genetic studies form the foundation of our knowledge on homologous recombination. Significant progress has also been made toward understanding the biochemical and biophysical properties of the proteins, complexes, and reaction intermediates involved in this essential DNA repair pathway. However, heterogeneous or transient recombination intermediates remain extremely difficult to assess through traditional ensemble methods, leaving an incomplete mechanistic picture of many steps that take place during homologous recombination. To help overcome some of these limitations, we have established DNA curtain methodologies as an experimental platform for studying homologous DNA recombination in real-time at the single-molecule level. Here, we present a detailed overview describing the preparation and use of single-stranded DNA curtains in applications related to the study of homologous DNA recombination with emphasis on recent work related to the study of the eukaryotic recombinase Rad51.

摘要

同源重组是参与双链DNA断裂修复的重要途径。遗传学研究构成了我们对同源重组认识的基础。在理解参与这一基本DNA修复途径的蛋白质、复合物和反应中间体的生化及生物物理特性方面也取得了重大进展。然而，通过传统的整体方法评估异质或瞬时重组中间体仍然极其困难，这使得同源重组过程中许多步骤的机制图景不完整。为了帮助克服其中一些限制，我们建立了DNA帘幕方法，作为在单分子水平实时研究同源DNA重组的实验平台。在此，我们提供了一份详细概述，描述了单链DNA帘幕在与同源DNA重组研究相关应用中的制备和使用，重点介绍了与真核重组酶Rad51研究相关的近期工作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/fb6c0d2008ea/nihms-1587874-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/7c186632c51d/nihms-1587874-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/10a1482e010b/nihms-1587874-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/d9b8f53d960c/nihms-1587874-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/283e347cedf7/nihms-1587874-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/9c22c31cd661/nihms-1587874-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/5b767e3acda2/nihms-1587874-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/fb6c0d2008ea/nihms-1587874-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/7c186632c51d/nihms-1587874-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/10a1482e010b/nihms-1587874-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/d9b8f53d960c/nihms-1587874-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/283e347cedf7/nihms-1587874-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/9c22c31cd661/nihms-1587874-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/5b767e3acda2/nihms-1587874-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f77/7229809/fb6c0d2008ea/nihms-1587874-f0007.jpg

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2
Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.大肠杆菌CRISPR-Cas系统对外源DNA的监测与处理
Cell. 2015 Nov 5;163(4):854-65. doi: 10.1016/j.cell.2015.10.003.
3
DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases.DNA重组。由Rad51/RecA重组酶家族进行的碱基三联体步移。
PRC1 组成和染色质相互作用的模块化定义了凝聚物的性质。
Mol Cell. 2024 May 2;84(9):1651-1666.e12. doi: 10.1016/j.molcel.2024.03.001. Epub 2024 Mar 22.
4
The separation pin distinguishes the pro- and anti-recombinogenic functions of Saccharomyces cerevisiae Srs2.分离钉区分了酿酒酵母 Srs2 的促进和抗重组功能。
Nat Commun. 2023 Dec 8;14(1):8144. doi: 10.1038/s41467-023-43918-4.
5
Structural insights into BCDX2 complex function in homologous recombination.结构洞察 BCDX2 复合物在同源重组中的功能。
Nature. 2023 Jul;619(7970):640-649. doi: 10.1038/s41586-023-06219-w. Epub 2023 Jun 21.
6
Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.单分子可视化研究 Pif1 解旋酶在单链 DNA 上的迁移。
J Biol Chem. 2023 Jun;299(6):104817. doi: 10.1016/j.jbc.2023.104817. Epub 2023 May 11.
7
Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.Rad54 和 Rdh54 防止 Srs2 介导的 Rad51 预联丝解体。
Proc Natl Acad Sci U S A. 2022 Jan 25;119(4). doi: 10.1073/pnas.2113871119.
8
Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks.双链断裂修复单分子研究的机制洞察
Front Cell Dev Biol. 2021 Nov 15;9:745311. doi: 10.3389/fcell.2021.745311. eCollection 2021.
9
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10
Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA.对单个受保护的DNA片段分子进行精确测序，以分析蛋白质在DNA上的分布和组装情况。
Chem Sci. 2021 Jan 2;12(6):2039-2049. doi: 10.1039/d0sc01742f.
Science. 2015 Aug 28;349(6251):977-81. doi: 10.1126/science.aab2666.
4
The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level.真核生物复制起始的动力学：单分子水平上的起始点特异性、许可和激活
Mol Cell. 2015 May 7;58(3):483-94. doi: 10.1016/j.molcel.2015.03.017. Epub 2015 Apr 23.
5
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Cold Spring Harb Perspect Biol. 2015 Mar 2;7(3):a016535. doi: 10.1101/cshperspect.a016535.
6
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Cell. 2015 Feb 26;160(5):856-869. doi: 10.1016/j.cell.2015.01.029. Epub 2015 Feb 12.
7
DNA-pairing and annealing processes in homologous recombination and homology-directed repair.同源重组和同源定向修复中的DNA配对与退火过程。
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8
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