Minami E, Ozeki Y, Matsuoka M, Koizuka N, Tanaka Y
Department of Pesticides, National Institute of Agro-Environmental Sciences, Tsukuba, Japan.
Eur J Biochem. 1989 Oct 20;185(1):19-25. doi: 10.1111/j.1432-1033.1989.tb15075.x.
A nearly full-length cDNA and a genomic clone were isolated that encoded the phenylalanine ammonia-lyase (PAL) of rice plants, and the complete nucleotide sequences were determined. The gene encodes a polypeptide of 701 amino acid residues. The deduced amino acid sequence is highly similar to that of PAL from Phaseolus vulgaris deduced from an incomplete cDNA fragment. The cloned gene spans 4412 bp and consists of two exons and one intron. The site of initiation of transcription was located -86 nucleotides (position 1) upstream from the translational initiation codon by the primer-extension method. Sequences analogous to TATA-box and GC-box were found in the 5'-upstream region from the transcriptional initiation site. Southern blot analysis showed that the PAL gene of rice plants exists as a small multi-gene family. Within this family, the genomic clone isolated in this study was shown to be responsive by light. This also indicated that this genomic sequence functions as a gene for phenylalanine ammonia-lyase in rice plants in vivo.
分离出了一个几乎全长的cDNA和一个基因组克隆，它们编码水稻植株的苯丙氨酸解氨酶（PAL），并测定了完整的核苷酸序列。该基因编码一个由701个氨基酸残基组成的多肽。推导的氨基酸序列与从一个不完整的cDNA片段推导的菜豆PAL的氨基酸序列高度相似。克隆的基因跨度为4412 bp，由两个外显子和一个内含子组成。通过引物延伸法确定转录起始位点位于翻译起始密码子上游86个核苷酸（位置1）处。在转录起始位点的5′上游区域发现了类似于TATA盒和GC盒的序列。Southern印迹分析表明，水稻植株的PAL基因以小多基因家族的形式存在。在这个家族中，本研究分离的基因组克隆显示对光有反应。这也表明该基因组序列在水稻植株体内作为苯丙氨酸解氨酶基因发挥作用。
