The College of Landscape Architecture, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji 133002, China.
Int J Mol Sci. 2022 Dec 10;23(24):15686. doi: 10.3390/ijms232415686.
Phenylalanine ammonia-lyase (PAL, E.C.4.3.1.5) catalyzes the benzene propane metabolism and is the most extensively studied enzyme of the phenylpropanoid pathway. However, the role of genes in , a non-model plant showing high capability toward abiotic stress, is less studied. Here, we cloned and found that it encodes a protein that resides in the cytoplasmic membrane. The mRNA of was strongly induced by NaCl or NaHCO treatment, especially in the root. Overexpressing in resulted in higher PAL enzyme activities, lower levels of malondialdehyde (MDA), and better root elongation in the seedlings under stress treatment compared to the control plants. The protective role of under saline-alkali stress was also observed in 30-day soil-grown plants, which showed higher levels of superoxide dismutase (SOD), proline, and chlorophyll compared to wild-type . Collectively, we provide evidence that is responsive to multiple abiotic stresses and that manipulating the expression of can be used to increase the tolerance to adverse environmental factors in plants.
苯丙氨酸解氨酶（PAL，E.C.4.3.1.5）催化苯丙烷代谢，是苯丙烷途径中研究最广泛的酶。然而，对于具有较强非生物胁迫能力的非模式植物 ，其基因的作用研究较少。本研究克隆了 ，并发现其编码的蛋白定位于细胞质膜。 的 mRNA 强烈诱导 NaCl 或 NaHCO 处理，特别是在根部。与对照植株相比，在胁迫处理下，过表达 在 中的幼苗具有更高的 PAL 酶活性、更低的丙二醛（MDA）水平和更好的根伸长。在 30 天的土壤种植植物中也观察到了 在盐碱性胁迫下的保护作用，其超氧化物歧化酶（SOD）、脯氨酸和叶绿素水平均高于野生型 。总之，我们提供的证据表明 对多种非生物胁迫有反应，并且操纵 的表达可用于提高植物对不利环境因素的耐受性。
