Shimoyama Atsuko, Fujisaka Aki, Obika Satoshi
Analytical Research Laboratories, Technology, Astellas Pharma Inc., 5-2-3 Tokodai, Tsukuba-Shi, Ibaraki 300-2698, Japan; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan; Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-Kita, Tondabayashi, Osaka 584-8540, Japan.
J Pharm Biomed Anal. 2017 Mar 20;136:55-65. doi: 10.1016/j.jpba.2016.12.036. Epub 2016 Dec 29.
We evaluated size exclusion chromatography (SEC) for the detection of high-order structure of phosphorothioate oligonucleotides (PS-oligo). Because of strong interaction between PS-oligo and column packing material, peaks were broader and elution time was longer than those of the corresponding natural DNA oligonucleotides. However, single- and double-stranded structures of PS-oligo were clearly separated and discriminated, while single-stranded with high-order structures such as G-quadruplex and hairpin structure were not distinguished from each other.
我们评估了尺寸排阻色谱法（SEC）用于检测硫代磷酸酯寡核苷酸（PS-寡核苷酸）的高级结构。由于PS-寡核苷酸与柱填充材料之间存在强相互作用，其峰比相应的天然DNA寡核苷酸更宽，洗脱时间更长。然而，PS-寡核苷酸的单链和双链结构能够清晰地分离和区分，而具有诸如G-四链体和发夹结构等高阶结构的单链之间则无法相互区分。
