将流体动力学色谱法扩展用于DNA片段大小测定和定量分析。
Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation.
作者信息
Wang Yanan, Zhou Yingyan, Zhang Dongtang, Wang Xiayan, Liu Shaorong
机构信息
Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, PR China.
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, 73019, USA.
出版信息
Heliyon. 2021 Sep 7;7(9):e07904. doi: 10.1016/j.heliyon.2021.e07904. eCollection 2021 Sep.
Hydrodynamic chromatography (HDC) is a technique originally developed for separating particles. We have recently extended it to DNA fragment sizing and quantitation. In this review, we focus on this extension. After we briefly introduce the history of HDC, we present the evolution of open tubular HDC for DNA fragment sizing. We cover both the theoretical aspect and the experimental implementation of this technique. We describe various approaches to execute the separation, discuss its representative applications and provide a future perspective of this technique in the conclusion section of this review.
流体动力学色谱法（HDC）是一种最初为分离颗粒而开发的技术。我们最近将其扩展到DNA片段大小测定和定量分析。在本综述中，我们重点关注这一扩展内容。在简要介绍HDC的历史之后，我们阐述了用于DNA片段大小测定的开管HDC的发展历程。我们涵盖了该技术的理论方面和实验实施。我们描述了进行分离的各种方法，讨论了其代表性应用，并在本综述的结论部分提供了该技术的未来展望。
Zotero 插件