Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan, ROC; Allergy & Clinical Immunology Research Center, National Cheng Kung University, Tainan 701, Taiwan, ROC; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan, ROC.
Department of Food Science, National Penghu University of Science and Technology, Penghu 880, Taiwan, ROC.
Biomaterials. 2017 Mar;120:185-194. doi: 10.1016/j.biomaterials.2016.12.022. Epub 2016 Dec 22.
Reactive oxygen species is the main contributor to photodynamic therapy. The results of this study show that a nitrogen-doped graphene quantum dot, serving as a photosensitizer, was capable of generating a higher amount of reactive oxygen species than a nitrogen-free graphene quantum dot in photodynamic therapy when photoexcited for only 3 min of 670 nm laser exposure (0.1 W cm), indicating highly improved antimicrobial effects. In addition, we found that higher nitrogen-bonding compositions of graphene quantum dots more efficiently performed photodynamic therapy actions than did the lower compositions that underwent identical treatments. Furthermore, the intrinsically emitted luminescence from nitrogen-doped graphene quantum dots and high photostability simultaneously enabled it to act as a promising contrast probe for tracking and localizing bacteria in biomedical imaging. Thus, the dual modality of nitrogen-doped graphene quantum dots presents possibilities for future clinical applications, and in particular multidrug resistant bacteria.
活性氧是光动力疗法的主要贡献者。这项研究的结果表明，在 670nm 激光照射仅 3 分钟（0.1W/cm）的情况下，作为光敏剂的氮掺杂石墨烯量子点在光动力疗法中比无氮石墨烯量子点产生了更高的活性氧，表明具有高度改善的抗菌效果。此外，我们发现，与经过相同处理的低氮结合组成的石墨烯量子点相比，具有更高氮结合组成的石墨烯量子点更有效地进行光动力疗法作用。此外，氮掺杂石墨烯量子点的本征发光和高光稳定性使其能够同时用作跟踪和定位生物医学成像中细菌的有前途的对比探针。因此，氮掺杂石墨烯量子点的双重模式为未来的临床应用提供了可能性，特别是对多药耐药菌。
