Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Bioresour Technol. 2017 Mar;228:193-200. doi: 10.1016/j.biortech.2016.12.081. Epub 2016 Dec 24.
Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, α-amylases, cellulases and pectinases. Among acid/alkaline treatment, ultrasound and microwave assisted methods, chemical treatments superiorly affected most of the studied substrates. Highest yields of produced proteases (50.5IUg) and α-amylases (50.75IUg) were achieved on alkaline treated corn pericarp. Alkaline treatment also promoted the secretion of cellulases on maize bran (1.19IUg). Highest yield of pectinases was obtained on untreated soybean meal (64.90IUg).
本研究旨在利用固态发酵（SSF）生产多种工业价值酶，因此对不同农业工业废物在支持新分离的芽孢杆菌 TMF-1 菌株生长方面的潜力进行了研究。由于初步研究的可行性得到了证实，因此进一步的目标包括评估几种研究中的农业副产品（豆粕、葵花粕、玉米麸皮、玉米皮、橄榄油渣和麦麸）的预处理方法对微生物生产力的影响，作为提高所产蛋白酶、α-淀粉酶、纤维素酶和果胶酶产量的手段。在酸/碱处理、超声和微波辅助方法中，化学处理对大多数研究的基质有较好的影响。经碱性处理的玉米皮可获得最高产蛋白酶（50.5IUg）和α-淀粉酶（50.75IUg）的产量。碱性处理还促进了玉米麸皮上纤维素酶的分泌（1.19IUg）。未经处理的豆粕可获得最高的果胶酶产量（64.90IUg）。
