Rees Jennifer, Haig David, Mack Victoria, Davis William C
School of Veterinary Medicine and Science, University of Nottingham, Sutton, Bonington, LE12 5RD, UK.
School of Veterinary Medicine and Science, University of Nottingham, Sutton, Bonington, LE12 5RD, UK.
Vet Immunol Immunopathol. 2017 Jan;183:40-44. doi: 10.1016/j.vetimm.2016.12.003. Epub 2016 Dec 6.
Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases.
流式细胞术用于鉴定识别仓鼠白细胞分化分子（hLDM）上保守表位的单克隆抗体（mAb），并对针对hLDM产生的单克隆抗体进行表征。对针对其他物种LDM产生的单克隆抗体进行初步筛选，得到了针对主要组织相容性（MHC）II分子、CD4和CD18的特异性单克隆抗体。对针对hLDM产生的多组单克隆抗体进行筛选，得到了22种新的单克隆抗体，包括针对MHC II分子的其他单克隆抗体以及识别所有白细胞、粒细胞、所有淋巴细胞、所有T细胞、一部分T细胞或所有B细胞上表达的LDM的单克隆抗体。通过流式细胞术（FC）检测，基于对所有仓鼠白细胞上表达的LDM表达模式与其他物种已知LDM表达模式的比较，预计有4种单克隆抗体可识别CD11a、CD44和CD45。对针对一部分仓鼠T细胞的特异性单克隆抗体与已知识别小鼠CD4的交叉反应性单克隆抗体以及一种识别CD8的单克隆抗体进行交叉比较，发现它们识别CD4。这些单克隆抗体的表征为利用仓鼠作为额外的模型物种来研究传染病免疫发病机制提供了更多机会。
