Davis William C, Hamilton Mary Jo
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA.
J Vet Sci. 2008 Mar;9(1):51-66. doi: 10.4142/jvs.2008.9.1.51.
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of the Eighth Human Leukocyte Differentiation Workshop yielded 7 additional mAbs. Screening of mAbs generated from mice immunized with leukocytes from rabbit thymus or spleen or concanavalin A activated peripheral blood and/or spleen lymphocytes has yielded 42 mAbs that recognize species restricted epitopes expressed on one or more lineages of leukocytes. Screening of the anti-rabbit mAbs against leukocytes from other species yielded one additional mAb. The studies show that screening of existing sets of mAbs for reactivity with rabbit LDM will not be productive and that a direct approach will be needed to develop mAbs for research in rabbits. The flow cytometric approach we developed to screen for mAbs of interest offers a way for individual laboratories to identify and characterize mAbs to LDM in rabbits and other species. A web-based program we developed provides a source of information that will facilitate analysis. It contains a searchable data base on known CD molecules and a data base on mAbs, known to react with LDM in one or more species of artiodactyla, equidae, carnivora, and or lagomorpha.
流式细胞术用于鉴定和表征与兔白细胞分化分子（LDM）反应的单克隆抗体（mAb）。针对其他物种的LDM开发的单克隆抗体筛选集，用于检测与兔LDM的反应性，结果产生了11种识别兔LDM直系同源物上保守表位的单克隆抗体，以及多种识别主要组织相容性复合体I类或II类分子上表达表位的单克隆抗体。对提交给第八届人类白细胞分化研讨会动物同源物分会的单克隆抗体进行筛选，又获得了7种单克隆抗体。用兔胸腺或脾脏白细胞或伴刀豆球蛋白A激活的外周血和/或脾脏淋巴细胞免疫小鼠产生的单克隆抗体进行筛选，得到了42种识别在一种或多种白细胞谱系上表达的物种限制性表位的单克隆抗体。针对来自其他物种的白细胞的抗兔单克隆抗体进行筛选，又获得了一种单克隆抗体。研究表明，筛选现有的单克隆抗体集与兔LDM的反应性不会有成效，需要一种直接的方法来开发用于兔研究的单克隆抗体。我们开发的用于筛选感兴趣的单克隆抗体的流式细胞术方法为各个实验室鉴定和表征兔及其他物种中针对LDM的单克隆抗体提供了一种途径。我们开发的基于网络的程序提供了一个信息源，将有助于分析。它包含一个关于已知CD分子的可搜索数据库和一个关于已知与偶蹄目、马科、食肉目和/或兔形目一种或多种物种中的LDM反应的单克隆抗体的数据库。
