去甲肾上腺素诱导平滑肌细胞多倍体化：第二信使的协同作用。

Noradrenaline induces the polyploidization of smooth muscle cells: the synergism of second messengers.

作者信息

Tjurmin A V, Rudchenko S A, Repin V S

机构信息

Institute of Experimental Cardiology, Academy of Medical Sciences, Moscow, USSR.

出版信息

Exp Cell Res. 1989 Oct;184(2):342-50. doi: 10.1016/0014-4827(89)90333-9.

DOI:10.1016/0014-4827(89)90333-9

PMID:2806397

Abstract

The effect of noradrenaline (NA) on DNA replication of cultured smooth muscle cells (SMC) isolated from rat aorta was examined. It was found that 10 microM NA significantly increased (approximately by twofold) the frequency of tetraploid cells. Cultivation of 4C cells isolated by flow cytometric cell sorting revealed that they were true polyploid cells. This receptor-mediated effect of NA was blocked only by simultaneous action of alpha- and beta-adrenoreceptor antagonists. SMC polyploidization was also stimulated by simultaneous application of direct activators of "second messenger" systems forskolin and phorbolmyristate-acetate. Thus, NA may be one of mediators of the "hypertensive" response of vessel wall SMC, which probably occurs due to synergism of two second messenger systems.

摘要

研究了去甲肾上腺素（NA）对从大鼠主动脉分离的培养平滑肌细胞（SMC）DNA复制的影响。发现10微摩尔NA显著增加（约两倍）四倍体细胞的频率。对通过流式细胞术细胞分选分离的4C细胞进行培养，结果显示它们是真正的多倍体细胞。NA的这种受体介导效应仅被α和β肾上腺素能受体拮抗剂同时作用所阻断。同时应用“第二信使”系统的直接激活剂福斯高林和佛波酯肉豆蔻酸酯-乙酸盐也可刺激SMC多倍体化。因此，NA可能是血管壁SMC“高血压”反应的介质之一，这种反应可能是由于两种第二信使系统的协同作用所致。