Printseva O Iu, Tiurmin A V
Kardiologiia. 1991 Dec;31(12):44-8.
The aortic intima and media isolated from hypertensives showed a significantly larger number (up to 20%) of smooth muscle cells (SMC) with tetraploid DNA content. The similar process was shown to be also a part of normal human vessel maturation. Normotensive human and rat aortic SMCs were found to accumulate 3H-thymidine, have a lower proliferative ability and they were apt to polyploidize in the primary culture. Such a population could not be detected in the aorta of spontaneously hypertensive rats. It was ascertained that 10 microM of noradrenaline significantly increased (approximately by 2 times) the occurrence of true polyploid cells in the rat aortic SMC subculture. The effect of noradrenaline was blocked by the concomitant effects of alpha- and beta-adrenoreceptor antagonists. SMC polyploidization was also stimulated by the simultaneously use of the direct activators of second messenger systems forskolin and phorbol-12-myristate-13-acetate. Thus, the SMC subpopulation that is apt to polyploidize exists in normal vessels and noradrenaline may be one of the mediators of a response of the vascular wall SMC, which seems to occur due to the synergism of two second messenger systems.
从高血压患者分离出的主动脉内膜和中膜显示，具有四倍体DNA含量的平滑肌细胞（SMC）数量显著增多（高达20%）。类似过程也被证明是正常人体血管成熟的一部分。研究发现，血压正常的人和大鼠主动脉平滑肌细胞能积累3H-胸腺嘧啶核苷，增殖能力较低，且在原代培养中易于多倍体化。在自发性高血压大鼠的主动脉中未检测到这样的细胞群。已确定，10微摩尔去甲肾上腺素能显著增加（约2倍）大鼠主动脉平滑肌细胞传代培养中真正多倍体细胞的出现率。去甲肾上腺素的作用被α和β肾上腺素能受体拮抗剂的联合作用所阻断。同时使用第二信使系统的直接激活剂福斯高林和佛波醇-12-肉豆蔻酸酯-13-乙酸酯也能刺激平滑肌细胞多倍体化。因此，易于多倍体化的平滑肌细胞亚群存在于正常血管中，而去甲肾上腺素可能是血管壁平滑肌细胞反应的介质之一，这似乎是由于两个第二信使系统的协同作用所致。
