Semple-Rowland S L, Ulshafer R J
Department of Ophthalmology, University of Florida, College of Medicine, Gainesville 32610-0284.
Exp Eye Res. 1989 Oct;49(4):665-75. doi: 10.1016/s0014-4835(89)80061-2.
The rd (retinal degeneration) chick possesses an autosomal recessive mutation which results in behavioral and electrophysiological blindness at hatch. Using the technique of two-dimensional gel electrophoresis, we have identified two groups of proteins whose expression in the retina/pigment epithelium/choroid (RET/PE/CH) of +/+, +/rd and rd/rd chicks is related to genotype. The two proteins within Group 1 had an apparent mass (Mr) of 63 kDa and isoelectric points (pI) of 6.48 and 6.55. The four proteins in Group 2 had an apparent Mr of 98 kDa and pI values ranging from 6.08 to 6.25. Quantities of the Group 1 proteins in RET/PE/CH of each of the three types of animals were found to be related to genotype; the amounts of each did not change with development. The expression of the Group 2 proteins in PET/PE/CH was found to change during retinal development. Proteins P98-6.08 (Mr-pI) and P98-6.13 were found in all rd/rd and +/rd RET/PE/CH and in most +/+ embryonic tissues. Proteins P98-6.19 and P98-6.25, which were present in the majority of +/+ embryonic and in all +/+ E21 (hatch RET/PE/CH, did not appear in +/rd tissues until hatch. P98-6.19 and P98-6.25 were never observed in rd/rd RET/PE/CH.
rd（视网膜变性）鸡具有常染色体隐性突变，导致孵化时出现行为和电生理失明。利用二维凝胶电泳技术，我们鉴定出两组蛋白质，其在+/+、+/rd和rd/rd鸡的视网膜/色素上皮/脉络膜（RET/PE/CH）中的表达与基因型相关。第1组中的两种蛋白质表观分子量（Mr）为63 kDa，等电点（pI）为6.48和6.55。第2组中的四种蛋白质表观Mr为98 kDa，pI值范围为6.08至6.25。发现这三种动物的RET/PE/CH中第1组蛋白质的量与基因型相关；每种蛋白质的量不会随发育而变化。发现第2组蛋白质在PET/PE/CH中的表达在视网膜发育过程中发生变化。在所有rd/rd和+/rd的RET/PE/CH以及大多数+/+胚胎组织中发现了蛋白质P98 - 6.08（Mr - pI）和P98 - 6.13。蛋白质P98 - 6.19和P98 - 6.25存在于大多数+/+胚胎以及所有+/+ E21（孵化时的RET/PE/CH）中，直到孵化才出现在+/rd组织中。在rd/rd的RET/PE/CH中从未观察到P98 - 6.19和P98 - 6.25。
