Tan Siran, Chi Qianru, Liu Tianqi, Sun Zhepeng, Min Yahong, Zhang Ziwei, Li Shu
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2017 Aug;178(2):301-309. doi: 10.1007/s12011-016-0924-8. Epub 2017 Jan 7.
To determine the negative effects of cadmium (Cd) exposure and the protective role of selenium (Se) on Cd-spiked neutrophils of chicken, forty-eight 28-day-old Isa Brown male chickens were divided randomly into four groups. Group I (control group) was fed with the basic diet containing 0.2 mg/kg Se. Group II (Se-treated group) was fed with the basic diet supplemented with NaSeO, and the total Se content was 2 mg/kg. Group III (Se/Cd-treated group) was fed with the basic diet supplemented with NaSeO; the total Se content was 2 mg/kg and supplemented with 150 mg/kg CdCl. Group IV (Cd-treated group) was fed with the basic diet supplemented with 150 mg/kg CdCl. Analyses of inflammatory factors, cytokines, and heat shock protein (Hsp) messenger RNA (mRNA) expression were detected by real-time PCR (RT-PCR). Additionally, we evaluated the phagocytic rate of neutrophils in peripheral blood. First, we observed that Cd significantly induced the mRNA expression levels of inflammatory factors NF-κB, iNOS, COX-2, and TNF-α, while Se/Cd treatment reduced their mRNA expression, although these expression levels remained higher than that of the control group. In addition, the mRNA expression levels of cytokines (IL-2, IL-4, and IL-10) for the Se-treated group exhibited significant differences between the Se/Cd-treated group and the Cd-treated group. Furthermore, the mRNA expression levels of Hsps demonstrated that the Se/Cd-treated group and the Cd-treated group were significantly higher (P < 0.05) than the control group and the Se-treated group. These results demonstrated that Se presented partial protection on Cd-spiked neutrophils of chicken with Hsps being involved in the process of the Cd-spiked toxic effects in chicken peripheral blood neutrophils.
为了确定镉（Cd）暴露的负面影响以及硒（Se）对鸡镉中毒中性粒细胞的保护作用，将48只28日龄的艾维茵褐壳雄性鸡随机分为四组。第一组（对照组）饲喂含0.2 mg/kg硒的基础日粮。第二组（硒处理组）饲喂添加亚硒酸钠的基础日粮，总硒含量为2 mg/kg。第三组（硒/镉处理组）饲喂添加亚硒酸钠的基础日粮，总硒含量为2 mg/kg，并添加150 mg/kg氯化镉。第四组（镉处理组）饲喂添加150 mg/kg氯化镉的基础日粮。通过实时荧光定量PCR（RT-PCR）检测炎症因子、细胞因子和热休克蛋白（Hsp）信使核糖核酸（mRNA）的表达。此外，我们评估了外周血中性粒细胞的吞噬率。首先，我们观察到镉显著诱导炎症因子NF-κB、诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和肿瘤坏死因子-α（TNF-α）的mRNA表达水平，而硒/镉处理降低了它们的mRNA表达，尽管这些表达水平仍高于对照组。此外，硒处理组细胞因子（白细胞介素-2、白细胞介素-4和白细胞介素-10）的mRNA表达水平在硒/镉处理组和镉处理组之间存在显著差异。此外，热休克蛋白的mRNA表达水平表明，硒/镉处理组和镉处理组显著高于对照组和硒处理组（P<0.05）。这些结果表明，硒对鸡镉中毒中性粒细胞具有部分保护作用，热休克蛋白参与了鸡外周血中性粒细胞镉中毒的毒性作用过程。
