Sun G X, Chen Y, Liu C P, Li S, Fu J
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biol Trace Elem Res. 2016 Aug;172(2):474-480. doi: 10.1007/s12011-015-0602-2. Epub 2016 Jan 4.
The possible beneficial role of selenium (Se) in heat shock proteins (HSPs) and inflammation damage induced by lead (Pb) in chickens is unclear. Therefore, the aim of this study was to investigate the effect of Se against Pb on the messenger RNA (mRNA) expression levels of HSPs (HSP 27, 40, 60, 70, and 90); heme oxygenase-1 (HO-1); and the inflammatory cytokines nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in the peripheral blood lymphocytes of chickens. A total of 360 1-day-old broiler chickens were randomly allocated into four groups (n = 90/group). The control group was fed a basic diet containing 0.2 mg/kg Se and 0.5 mg/kg Pb; the Se supplementation group (+Se group) was fed a Se-adequate (sodium selenite) diet containing 1 mg/kg Se and 0.5 mg/kg Pb; the Pb-supplemented group (+Pb group) was fed a Pb acetate diet containing 0.2 mg/kg Se and 350 mg/kg Pb; and the Se and Pb compound group (Se + Pb group) was fed a diet containing 1 mg/kg Se and 350 mg/kg Pb. The blood was collected and examined for the mRNA levels of HSP and inflammatory cytokine genes at 30 and 60 days old. The results showed that Pb poisoning induced the mRNA expression of HSPs and inflammatory cytokines in the peripheral blood lymphocytes of chickens. In addition, Se alleviated the Pb-induced increase in HSP and inflammatory cytokine mRNA levels in chicken peripheral blood lymphocytes. In conclusion, Se can antagonize the toxic effects of Pb on chickens and protect the chickens' peripheral blood lymphocytes in normal physiological function.
硒（Se）在鸡热休克蛋白（HSPs）以及铅（Pb）诱导的炎症损伤中可能发挥的有益作用尚不清楚。因此，本研究旨在探讨硒对铅所致鸡外周血淋巴细胞中热休克蛋白（HSP 27、40、60、70和90）、血红素加氧酶-1（HO-1）的信使核糖核酸（mRNA）表达水平，以及炎性细胞因子核因子κB（NF-κB）、肿瘤坏死因子α（TNF-α）、环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的影响。总共360只1日龄肉鸡被随机分为四组（每组n = 90）。对照组饲喂含0.2 mg/kg硒和0.5 mg/kg铅的基础日粮；硒补充组（+Se组）饲喂含1 mg/kg硒和0.5 mg/kg铅的硒充足（亚硒酸钠）日粮；铅补充组（+Pb组）饲喂含0.2 mg/kg硒和350 mg/kg醋酸铅的日粮；硒和铅复合组（Se + Pb组）饲喂含1 mg/kg硒和350 mg/kg铅的日粮。在30日龄和60日龄时采集血液，检测热休克蛋白和炎性细胞因子基因的mRNA水平。结果表明，铅中毒诱导了鸡外周血淋巴细胞中热休克蛋白和炎性细胞因子的mRNA表达。此外，硒减轻了铅诱导的鸡外周血淋巴细胞中热休克蛋白和炎性细胞因子mRNA水平的升高。总之，硒可以拮抗铅对鸡的毒性作用，并保护鸡外周血淋巴细胞的正常生理功能。
