Spring P M, Hoff H F
Department of Atherosclerosis Research, Cleveland Clinic Foundation, Ohio 44195.
Exp Mol Pathol. 1989 Oct;51(2):179-85. doi: 10.1016/0014-4800(89)90018-x.
We had previously used an electrophoretic transfer procedure to determine the topographic distribution of low density lipoprotein (LDL) accumulation in the aortic intima of normolipemic swine. In this present study we have employed a similar procedure to assess whether LDL-rich sites consistently demonstrate increased intimal thickening at the iliac bifurcation and common iliac arteries. The topographic distribution of LDL-rich sites was determined in the aortas of six subjects ranging in age from 16 to 36 years, by transferring LDL by electrophoresis from the tissue into an agarose gel containing anti-LDL, and then staining the immunofixed LDL in the gel for lipid. LDL-rich sites were found in all but two of these cases. On the basis of control studies establishing the level of nonspecific staining, we determined that the cutoff between LDL-rich and LDL-poor zones was 37 mg apoB protein/mm2 intimal surface area. Intimal thickening was found to be threefold greater in LDL-rich than in LDL-poor regions. These results confirm and extend earlier immunohistochemical studies suggesting a preferential accumulation of LDL at sites of intimal thickening in human arteries.
我们之前曾使用一种电泳转移程序来确定正常血脂猪主动脉内膜中低密度脂蛋白（LDL）积累的地形分布。在本研究中，我们采用了类似的程序来评估富含LDL的部位是否始终在髂动脉分叉处和髂总动脉处表现出内膜增厚增加。通过将LDL通过电泳从组织转移到含有抗LDL的琼脂糖凝胶中，然后对凝胶中免疫固定的LDL进行脂质染色，确定了6名年龄在16至36岁之间受试者主动脉中富含LDL部位的地形分布。在这些病例中，除两例外，其他病例均发现了富含LDL的部位。基于确定非特异性染色水平的对照研究，我们确定富含LDL区域和LDL贫乏区域之间的界限为内膜表面积37 mg载脂蛋白B蛋白/mm²。发现富含LDL区域的内膜增厚比LDL贫乏区域大两倍。这些结果证实并扩展了早期的免疫组织化学研究，表明LDL在人类动脉内膜增厚部位优先积累。
