Sanglard D, Fiechter A
Department of Biotechnology, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich.
FEBS Lett. 1989 Oct 9;256(1-2):128-34. doi: 10.1016/0014-5793(89)81732-6.
The reexamination of a genomic lambda gt11 Candida tropicalis expression library for the presence of genes related to the previously reported alkane-inducible cytochrome P450alk gene (P450alk), which is the first member of the P450LII gene family, was undertaken. A positive clone with a DNA fragment having 69% similarity with a portion of P450alk was isolated. As in the case of P450alk, this new putative P450 gene was also induced by tetradecane when C. tropicalis was grown on this carbon source and was therefore named P450alk2, P450alk1 corresponding to the first isolated P450 gene. In addition to P450alk2, the existence of other P450alk-related genes is suggested by the hybridization pattern of P450alk1 and P450alk2 probes with the C. tropicalis genomic DNA. The P450LII gene family in C. tropicalis appears therefore to include several different members. This heterogeneity is presently a unique feature within yeast P450 gene families and resembles the situation existing in P450 gene families of higher eukaryotes.
对一个基因组λgt11热带假丝酵母表达文库进行重新检测，以寻找与先前报道的烷烃诱导型细胞色素P450alk基因（P450alk）相关的基因，P450alk是P450LII基因家族的首个成员。分离出一个阳性克隆，其DNA片段与P450alk的一部分具有69%的相似性。与P450alk的情况一样，当热带假丝酵母在十四烷作为碳源的条件下生长时，这个新的假定P450基因也会被十四烷诱导，因此被命名为P450alk2，P450alk1则对应第一个分离出的P450基因。除了P450alk2之外，P450alk1和P450alk2探针与热带假丝酵母基因组DNA的杂交模式表明还存在其他与P450alk相关的基因。因此，热带假丝酵母中的P450LII基因家族似乎包括几个不同的成员。这种异质性目前是酵母P450基因家族中的一个独特特征，类似于高等真核生物P450基因家族中的情况。
