Sanglard D, Chen C, Loper J C
Biochem Biophys Res Commun. 1987 Apr 14;144(1):251-7. doi: 10.1016/s0006-291x(87)80503-x.
The gene for the alkane-inducible cytochrome P450, P450alk, has been isolated from the yeast Candida tropicalis by immunoscreening a lambda gt11 library. Isolation of the gene has been identified on the basis of its inducibility and partial DNA sequence. Transcripts of this gene were induced by alkane to levels 500 to 1000 fold over those detected in glucose-grown cells. The nucleotide sequence of the 3' portion of this gene revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
通过对λgt11文库进行免疫筛选，从热带假丝酵母中分离出了可被烷烃诱导的细胞色素P450（P450alk）的基因。该基因的分离是基于其可诱导性和部分DNA序列确定的。该基因的转录本在烷烃诱导下的水平比在葡萄糖培养细胞中检测到的水平高500至1000倍。该基因3'端部分的核苷酸序列揭示了一个与P450基因家族特征性的血红素结合片段相对应的编码序列。
