Yu Zehui, Geng Yi, Huang Anming, Wang Kaiyu, Huang Xiaoli, Chen Defang, Ou Yangping, Wang Jun
College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
J Invertebr Pathol. 2017 Mar;144:32-36. doi: 10.1016/j.jip.2017.01.001. Epub 2017 Jan 6.
A novel p38 MAPK gene from S. paramamosain was cloned and characterized by rapid amplification of cDNA ends (RACE) technology. S. paramamosain p38 (Sp-p38) MAPK gene consists of an open reading frame of 1095bp encoding a 365-amino-acid protein, which showed close phylogenetic relationship to Litopenaeus vannamei p38 MAPK. The tissue distribution patterns showed that Sp-p38 MAPK was widely expressed in all examined tissues, with the highest expression in hemocytes and intestines. The expression levels of Sp-p38 MAPK in hemocytes was up-regulated post-stimulation, which reached the peak at 6h and 12h after bacteria (S. aureus and V. harveyi) and WSSV infection, respectively. In conclusion, our data contributed to define the biological characteristics of Sp-p38 MAPK and further demonstrated the critical role of Sp-p38 MAPK in vivo during the viral and bacterial infection.
通过cDNA末端快速扩增（RACE）技术克隆并鉴定了来自拟穴青蟹的一个新的p38丝裂原活化蛋白激酶（MAPK）基因。拟穴青蟹p38（Sp-p38）MAPK基因由一个1095bp的开放阅读框组成，编码一个365个氨基酸的蛋白质，其与凡纳滨对虾p38 MAPK显示出密切的系统发育关系。组织分布模式表明，Sp-p38 MAPK在所有检测组织中广泛表达，在血细胞和肠道中表达最高。Sp-p38 MAPK在血细胞中的表达水平在刺激后上调，分别在细菌（金黄色葡萄球菌和哈维氏弧菌）和白斑综合征病毒感染后6小时和12小时达到峰值。总之，我们的数据有助于确定Sp-p38 MAPK的生物学特性，并进一步证明了Sp-p38 MAPK在病毒和细菌感染期间在体内的关键作用。
