Etv2是造血内皮谱系的主要调节因子。
Etv2 IS A MASTER REGULATOR OF HEMATOENDOTHELIAL LINEAGES.
作者信息
Garry Daniel J
机构信息
MINNEAPOLIS, MINNESOTA.
出版信息
Trans Am Clin Climatol Assoc. 2016;127:212-223.
Abstract
Ets transcription factors are important developmental regulators and have been shown to function as modulators of cell fate. We have previously discovered Ets variant 2 (Etv2) as an essential regulator of the hematoendothelial lineage. We have demonstrated that mutant embryos are non-viable and lack hematoendothelial lineages. We have utilized gene editing technologies to define upstream regulators of the gene and we and others have defined downstream target genes. Recent studies have demonstrated that Etv2, in combination with co-factors, promote a hematoendothelial fate in differentiating human-induced pluripotent stem cells. Collectively, these studies support the notion that Etv2 is a master regulator of the hematoendothelial lineages. Definition of these master regulators and the use of emerging technologies will provide a platform for engineering large animal models that will be useful for clinical research and regenerative medicine and will potentially impact the treatment of chronic vascular diseases.
摘要
Ets转录因子是重要的发育调节因子，已被证明可作为细胞命运的调节因子。我们之前已发现Ets变体2（Etv2）是血液内皮谱系的关键调节因子。我们已证明，突变胚胎无法存活且缺乏血液内皮谱系。我们利用基因编辑技术来确定该基因的上游调节因子，并且我们和其他人已经确定了下游靶基因。最近的研究表明，Etv2与辅助因子共同作用，可促进分化中的人诱导多能干细胞形成血液内皮命运。总体而言，这些研究支持了Etv2是血液内皮谱系的主要调节因子这一观点。确定这些主要调节因子并利用新兴技术将为构建大型动物模型提供一个平台，这将有助于临床研究和再生医学，并且可能会影响慢性血管疾病的治疗。
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Cell Rep. 2015 Nov 3;13(5):915-23. doi: 10.1016/j.celrep.2015.09.060. Epub 2015 Oct 22.
2
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J Biol Chem. 2015 Nov 20;290(47):28107-28119. doi: 10.1074/jbc.M115.662197. Epub 2015 Sep 22.
3
Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2.由ETS转录因子ER71/ETV2精心编排的造血和内皮细胞程序的诱导。
EMBO Rep. 2015 May;16(5):654-69. doi: 10.15252/embr.201439939. Epub 2015 Mar 23.
4
The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.转录因子Mesp1与环磷酸腺苷反应元件结合蛋白1（Creb1）相互作用，并共同激活Ets变体2（Etv2）的基因表达。
J Biol Chem. 2015 Apr 10;290(15):9614-25. doi: 10.1074/jbc.M114.614628. Epub 2015 Feb 18.
5
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Stem Cells Dev. 2014 Sep 1;23(17):2004-13. doi: 10.1089/scd.2013.0525. Epub 2014 Jun 17.
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