Brumsted J R, Chapitis J, Deaton J L, Riddick D H, Gibson M
Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington 05405-0068.
Fertil Steril. 1989 Nov;52(5):769-73.
Differences in prostaglandin (PG) F2 alpha synthesis and degradation were sought between early luteal endometrium (histology day 15 to 22, n = 6) and late luteal endometrium (histology day 23 to 28, n = 6). In addition, alterations in PGF2 alpha synthesis and degradation in response to 17 beta-estradiol (E2) and progesterone (P) were examined to clarify the mechanism of steroid modulation of endometrial PG production (n = 12). Endometrium was maintained in tissue culture and the concentration of PGF2 alpha and 13,14 dehydro-15 keto F2 alpha (DHKF2 alpha) was determined in spent media by radioimmunoassay. Prostaglandin F2 alpha and DHKF2 alpha output from luteal endometrium exposed to P and E2 + P were both significantly reduced when compared with no addition or E2 treatments. This implies that the modulation of PGF2 alpha output by P in vitro is secondary to altered synthesis. There was an increase in PGF2 alpha output from late luteal endometrium when compared with early luteal endometrium in the P and E2 + P treatments, but DHKF2 alpha remained unchanged. These data imply that the temporal increase in PGF2 alpha output is the result of alterations in both PG synthesis and catabolism.
研究了早期黄体期子宫内膜（组织学第15至22天，n = 6）和晚期黄体期子宫内膜（组织学第23至28天，n = 6）之间前列腺素（PG）F2α合成与降解的差异。此外，研究了17β-雌二醇（E2）和孕酮（P）对PGF2α合成与降解的影响，以阐明甾体激素调节子宫内膜PG生成的机制（n = 12）。将子宫内膜置于组织培养中，通过放射免疫测定法测定培养液中PGF2α和13,14-脱氢-15-酮F2α（DHKF2α）的浓度。与未添加或E2处理相比，暴露于P和E2 + P的黄体期子宫内膜中PGF2α和DHKF2α的输出均显著降低。这表明体外P对PGF2α输出的调节是合成改变的继发结果。在P和E2 + P处理中，与早期黄体期子宫内膜相比，晚期黄体期子宫内膜中PGF2α的输出增加，但DHKF2α保持不变。这些数据表明PGF2α输出的时间性增加是PG合成和分解代谢改变的结果。
