虾的转录组组装
transcriptome assembly of shrimp .
作者信息
Perina Alejandra, González-Tizón Ana M, Meilán Iago F, Martínez-Lage Andrés
机构信息
Department of Cell and Molecular Biology, Evolutionary Biology Group (GIBE), Universidade da Coruña, A Fraga 10, E-15008 A Coruña, Spain; Centro de Investigaciones Científicas Avanzadas (CICA), Universidade da Coruña, 15071 A Coruña, Spain.
aCore, Falperra 36, E-15005 A Coruña, Spain.
出版信息
Genom Data. 2016 Dec 23;11:89-91. doi: 10.1016/j.gdata.2016.12.009. eCollection 2017 Mar.
UNLABELLED
The shrimp is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of . RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis.
SPECIFICATIONS
[Table: see text].
未标记
虾是一种具有高商业价值的沿海十足目甲壳类动物。它被捕捞以供人类食用。在本研究中，我们使用Illumina测序技术（HiSeq 2000）对虾的转录组进行测序、组装和注释。从成年个体的肌肉以及一组幼虫中分离RNA。使用TruSeq RNA样本制备试剂盒v2构建了总共4个cDNA文库。本研究中的原始数据已存入NCBI SRA数据库，研究登录号为SRP090769。使用Trinity软件对获得的数据进行转录组组装，并通过TransDecoder预测编码区域。我们使用Blastp和Sma3s对鉴定出的蛋白质进行注释。转录组数据可为了解参与幼虫发育和变态的基因提供一些见解。
规格
[表格：见正文]
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