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本文引用的文献

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The detection of EGFR mutation status in plasma is reproducible and can dynamically predict the efficacy of EGFR-TKI.血浆中表皮生长因子受体（EGFR）突变状态的检测具有可重复性，并且能够动态预测表皮生长因子受体酪氨酸激酶抑制剂（EGFR-TKI）的疗效。
Thorac Cancer. 2012 Nov;3(4):334-340. doi: 10.1111/j.1759-7714.2012.00133.x.
2
Implications of Blood-Based T790M Genotyping and Beyond in Epidermal Growth Factor Receptor-Mutant Non-Small-Cell Lung Cancer.基于血液的T790M基因分型在表皮生长因子受体突变的非小细胞肺癌中的意义及其他影响
J Clin Oncol. 2016 Oct 1;34(28):3361-2. doi: 10.1200/JCO.2016.68.3458. Epub 2016 Jul 18.
3
Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies.游离DNA（cfDNA）：新兴技术塑造的癌症临床意义及应用
Mol Cancer Res. 2016 Oct;14(10):898-908. doi: 10.1158/1541-7786.MCR-16-0044. Epub 2016 Jul 15.
4
Association Between Plasma Genotyping and Outcomes of Treatment With Osimertinib (AZD9291) in Advanced Non-Small-Cell Lung Cancer.晚期非小细胞肺癌患者血浆基因分型与奥希替尼（AZD9291）治疗结果的相关性
J Clin Oncol. 2016 Oct 1;34(28):3375-82. doi: 10.1200/JCO.2016.66.7162. Epub 2016 Jun 27.
5
Characterization of 298 Patients with Lung Cancer Harboring MET Exon 14 Skipping Alterations.298 例携带 MET 外显子 14 跳跃改变的肺癌患者的特征。
J Thorac Oncol. 2016 Sep;11(9):1493-502. doi: 10.1016/j.jtho.2016.06.004. Epub 2016 Jun 22.
6
The International Association for the Study of Lung Cancer Consensus Statement on Optimizing Management of EGFR Mutation-Positive Non-Small Cell Lung Cancer: Status in 2016.国际肺癌研究协会关于优化 EGFR 突变阳性非小细胞肺癌管理的共识声明：2016 年现状。
J Thorac Oncol. 2016 Jul;11(7):946-63. doi: 10.1016/j.jtho.2016.05.008. Epub 2016 May 23.
7
Circulating DNA addresses cancer monitoring in non small cell lung cancer patients for detection and capturing the dynamic changes of the disease.循环DNA用于非小细胞肺癌患者的癌症监测，以检测和捕捉疾病的动态变化。
Springerplus. 2016 Apr 26;5:531. doi: 10.1186/s40064-016-2141-5. eCollection 2016.
8
Extracellular miR-1246 promotes lung cancer cell proliferation and enhances radioresistance by directly targeting DR5.细胞外miR-1246通过直接靶向DR5促进肺癌细胞增殖并增强放射抗性。
Oncotarget. 2016 May 31;7(22):32707-22. doi: 10.18632/oncotarget.9017.
9
Integrated digital error suppression for improved detection of circulating tumor DNA.用于改善循环肿瘤DNA检测的集成数字误差抑制
Nat Biotechnol. 2016 May;34(5):547-555. doi: 10.1038/nbt.3520. Epub 2016 Mar 28.
10
Concordance of anaplastic lymphoma kinase (ALK) gene rearrangements between circulating tumor cells and tumor in non-small cell lung cancer.非小细胞肺癌中循环肿瘤细胞与肿瘤组织间间变性淋巴瘤激酶（ALK）基因重排的一致性
Oncotarget. 2016 Apr 26;7(17):23251-62. doi: 10.18632/oncotarget.8136.

非小细胞肺癌中的液体活检

Liquid Biopsy in Non-Small Cell Lung Cancer.

作者信息

Molina-Vila Miguel A, Mayo-de-Las-Casas Clara, Giménez-Capitán Ana, Jordana-Ariza Núria, Garzón Mónica, Balada Ariadna, Villatoro Sergi, Teixidó Cristina, García-Peláez Beatriz, Aguado Cristina, Catalán María José, Campos Raquel, Pérez-Rosado Ana, Bertran-Alamillo Jordi, Martínez-Bueno Alejandro, Gil María-de-Los-Llanos, González-Cao María, González Xavier, Morales-Espinosa Daniela, Viteri Santiago, Karachaliou Niki, Rosell Rafael

机构信息

Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.

Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.

出版信息

Front Med (Lausanne). 2016 Dec 23;3:69. doi: 10.3389/fmed.2016.00069. eCollection 2016.

DOI:10.3389/fmed.2016.00069
PMID:28066769
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5179978/
Abstract

Liquid biopsy analyses are already incorporated in the routine clinical practice in many hospitals and oncology departments worldwide, improving the selection of treatments and monitoring of lung cancer patients. Although they have not yet reached its full potential, liquid biopsy-based tests will soon be as widespread as "standard" biopsies and imaging techniques, offering invaluable diagnostic, prognostic, and predictive information. This review summarizes the techniques available for the isolation and analysis of circulating free DNA and RNA, exosomes, tumor-educated platelets, and circulating tumor cells from the blood of cancer patients, presents the methodological challenges associated with each of these materials, and discusses the clinical applications of liquid biopsy testing in lung cancer.

摘要

液体活检分析已被纳入全球许多医院和肿瘤科室的常规临床实践中，改善了肺癌患者的治疗选择和监测。尽管它们尚未发挥出全部潜力，但基于液体活检的检测很快将与“标准”活检和成像技术一样广泛应用，提供宝贵的诊断、预后和预测信息。本综述总结了从癌症患者血液中分离和分析循环游离DNA和RNA、外泌体、肿瘤诱导血小板和循环肿瘤细胞的可用技术，介绍了与这些材料相关的方法学挑战，并讨论了液体活检检测在肺癌中的临床应用。