Wang C, Zhu Y, Li F, Huang L
Department of Animal Science, Shandong Agricultural University, Taian, Shandong, China.
J Anim Physiol Anim Nutr (Berl). 2017 Oct;101(5):e1-e13. doi: 10.1111/jpn.12629. Epub 2017 Jan 8.
The objective of this experiment was to investigate the effect of Lactobacillus isolates on intestinal bacterial community composition of growing Rex Rabbits. A total of 120 weaned Rex Rabbits (30 days old, 30 per group) were used for the experiment, which started after an adaptation period of 7 days. The control group was fed with basal diet only, while the treatment I, II and III groups were fed with basal diet adding antibiotics, Lactobacillus zeae (LB1) and Lactobacillus casei (L3) respectively. Growth performance, immune response and intestinal flora have been examined. The results obtained were as follows: (i) F/G of the rabbits fed with Lactobacillus isolates was significantly lower than that of the control group (p < 0.05). (ii) The concentration of ALT decreased significantly (p < 0.05) and that of IgG and IgM increased significantly (p < 0.05) after feeding rabbits with Lactobacillus isolates. (iii) Lactobacillus isolates had no influence on the number of mast cells in duodenum and jejunum, but increased the number of mast cells in caecum significantly (p < 0.05). (iv) The data from pyrosequencing-based analysis suggested that the bacterial community in the rabbit's intestinal flora can be changed by Lactobacillus isolates and antibiotics, especially for the microbial diversity and abundance in the caecum. In conclusion, the application of proper Lactobacillus isolates can improve the growth performance, enhance the immunological function and adjust the intestinal micro-ecosystem of growing Rex Rabbits.
本实验的目的是研究分离的乳酸杆菌对生长中的獭兔肠道细菌群落组成的影响。总共120只断奶獭兔（30日龄，每组30只）用于该实验，实验在7天的适应期后开始。对照组仅饲喂基础日粮，而处理I、II和III组分别饲喂添加抗生素、玉米乳酸杆菌（LB1）和干酪乳杆菌（L3）的基础日粮。检测了生长性能、免疫反应和肠道菌群。获得的结果如下：（i）饲喂分离的乳酸杆菌的兔子的料肉比显著低于对照组（p<0.05）。（ii）给兔子饲喂分离的乳酸杆菌后，谷丙转氨酶浓度显著降低（p<0.05），免疫球蛋白G和免疫球蛋白M浓度显著升高（p<0.05）。（iii）分离的乳酸杆菌对十二指肠和空肠中的肥大细胞数量没有影响，但显著增加了盲肠中的肥大细胞数量（p<0.05）。（iv）基于焦磷酸测序分析的数据表明，分离的乳酸杆菌和抗生素可改变獭兔肠道菌群中的细菌群落，尤其是盲肠中的微生物多样性和丰度。总之，应用合适的分离乳酸杆菌可提高生长中獭兔的生长性能，增强免疫功能并调节肠道微生态系统。
