Zou Fuqin, Zeng Dong, Wen Bin, Sun Hao, Zhou Yi, Yang Mingyue, Peng Zhirong, Xu Shuai, Wang Hesong, Fu Xiangchao, Du Dan, Zeng Yan, Zhu Hui, Pan Kangcheng, Jing Bo, Wang Ping, Ni Xueqin
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, Sichuan, China.
AMB Express. 2016 Dec;6(1):100. doi: 10.1186/s13568-016-0273-1. Epub 2016 Oct 21.
Antibiotics have been widely used for the prevention and the treatment of diseases to humans and animals, and they have fed additives for agricultural animals to promote growth. However, there is a growing concern over the practice due to its side effects on intestinal microbial communities which plays a vital role in animals' health. To investigate the effect of antibiotics on the bacterial population of the caecum in rex rabbits, 80 rex rabbits were randomly divided into four groups: control group (B, basal diet), chlortetracycline group (C, 50 mg/kg), colistin sulfate group (S, 20 mg/kg) and zinc bacitracin group (Z, 40 mg/kg). Caecum microbial communities of rex rabbits from the four groups were analyzed through Illumina Miseq platform after being fed 28 days. The results showed that most obtained sequences belongs to Firmicutes followed by Bacteroidetes, and the ratio of Bacteroidetes/Firmicutes in C group (42.31 %) was higher than that in Z group (21.84 %). Zinc bacitracin supplementation caused a significant decreased of the Proteobacteria phylum and Lactobacillus spp. (P < 0.05), while the Lactobacillus spp. significantly increased in S group (P < 0.05). In addition, Ruminococcus spp., especially Ruminococcus albus were the predominant bacterial species found in both S and Z groups. The proportion of Coprococcus spp. significantly increased in Z group (P < 0.05). These findings suggested that the antibiotics used may cause significant changes in the caecum microbiota of rex rabbits, and we also found C group had a similarity caecum bacteria structure with B group which was probably due to the high levels of chlortetracycline resistance.
抗生素已被广泛用于预防和治疗人类和动物疾病，并且被用作农业动物的饲料添加剂以促进生长。然而，由于其对肠道微生物群落有副作用，而肠道微生物群落在动物健康中起着至关重要的作用，因此人们对这种做法的担忧日益增加。为了研究抗生素对獭兔盲肠细菌种群的影响，80只獭兔被随机分为四组：对照组（B，基础日粮）、金霉素组（C，50毫克/千克）、硫酸黏菌素组（S，20毫克/千克）和杆菌肽锌组（Z，40毫克/千克）。四组獭兔在喂养28天后，通过Illumina Miseq平台分析其盲肠微生物群落。结果表明，大多数获得的序列属于厚壁菌门，其次是拟杆菌门，C组（42.31%）的拟杆菌门/厚壁菌门比例高于Z组（21.84%）。补充杆菌肽锌导致变形菌门和乳酸杆菌属显著减少（P<0.05），而S组的乳酸杆菌属显著增加（P<0.05）。此外，瘤胃球菌属，尤其是白色瘤胃球菌是在S组和Z组中发现的主要细菌种类。Z组中粪球菌属的比例显著增加（P<0.05）。这些发现表明，所使用的抗生素可能会导致獭兔盲肠微生物群发生显著变化，并且我们还发现C组与B组的盲肠细菌结构相似，这可能是由于金霉素抗性水平较高所致。
