Penninger J, Klima J, Kroemer G, Dietrich H, Hala K, Wick G
Institute for General and Experimental Pathology, Medical School, University of Innsbruck, Austria.
Dev Comp Immunol. 1989 Fall;13(4):313-27. doi: 10.1016/0145-305x(89)90041-4.
Thymic nurse cells (TNC) are multicellular complexes composed of morphologically intact thymocytes internalized within epithelial cells. Due to their unusual features, they have been suspected to provide an appropriate microenvironment for T-cell repertoire acquisition. In recent functional studies in the chicken system, we have shown that intra-thymic nurse cell-lymphocytes (TNC-L) are highly efficient inducers of a graft-versus-host reaction (GvHR) in an allogeneic chorionallantoic membrane (CAM) assay, manifested by the formation of visible colonies, so-called pocks. Immunohistochemical and electron microscopical assessment of pocks revealed no differences between the cellular components and ultrastructural features of GvHR induced by TNC-L or peripheral blood lymphocytes (PBL). In addition, the stimulation of this local GvHR by interleukin 2 (IL-2) was demonstrated.
胸腺哺育细胞（TNC）是由内化于上皮细胞内的形态完整的胸腺细胞组成的多细胞复合体。由于其独特的特征，人们怀疑它们为T细胞库的获得提供了适宜的微环境。在最近对鸡系统的功能研究中，我们发现在同种异体绒毛尿囊膜（CAM）试验中，胸腺内哺育细胞淋巴细胞（TNC-L）是移植物抗宿主反应（GvHR）的高效诱导剂，表现为形成可见的菌落，即所谓的痘疱。对痘疱进行免疫组织化学和电子显微镜评估发现，TNC-L或外周血淋巴细胞（PBL）诱导的GvHR的细胞成分和超微结构特征之间没有差异。此外，还证实了白细胞介素2（IL-2）对这种局部GvHR的刺激作用。
