Zollinger L, Potworowski E F
Transplantation. 1979 Aug;28(2):100-2. doi: 10.1097/00007890-197908000-00005.
Two thymic stromal fractions previously shown to have specific enhancing effects on anatomically distinct T cell populations were tested for their capacity to induce functionally distinct T cell subsets. Parental mice were injected with either soluble thymic fraction (STF) or insoluble thymic fraction (ITF), and their lymphoid cells were harvested 11 days later. It was shown that ITF elicited a splenic corticosensitive T cell subset endowed with enhanced graft-versus-host reaction (GVHR)-inducing capacity. On the other hand, STF increased, mainly in lymph nodes, the number of corticoresistant T cells significantly less active in GVHR. Furthermore, lymphocytes from ITF-treated parental donors could become corticoresistant with reduced GVHR activity after a 1-hr in vitro incubation with STF. We have thus shown that two different elements of the thymic microenvironment could modulate the intensity of the GVHR by modifying the equilibrium between two T cell subsets. These are believed to represent two consecutive differentiation stages.
先前已证明对解剖学上不同的T细胞群体具有特定增强作用的两种胸腺基质组分，被测试其诱导功能上不同的T细胞亚群的能力。给亲代小鼠注射可溶性胸腺组分（STF）或不溶性胸腺组分（ITF），11天后收获它们的淋巴细胞。结果显示，ITF诱导出一种脾皮质敏感T细胞亚群，其具有增强的移植物抗宿主反应（GVHR）诱导能力。另一方面，STF主要在淋巴结中增加了皮质抗性T细胞的数量，这些细胞在GVHR中的活性明显较低。此外，来自ITF处理的亲代供体的淋巴细胞在与STF进行1小时体外孵育后，可转变为皮质抗性且GVHR活性降低。因此，我们已经表明，胸腺微环境的两种不同成分可通过改变两个T细胞亚群之间的平衡来调节GVHR的强度。据信这两个亚群代表两个连续的分化阶段。
