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从土耳其采集的苔藓(Cinclidotus fontinaloides (Hedw.) P.Beauv. 和 Palustriella commutata (Hedw.) Ochyra)的化学成分、抗菌、抗氧化和花青素活性

Chemical composition, antimicrobial, antioxidant and anthocyanin activities of mosses (Cinclidotus fontinaloides (Hedw.) P.Beauv. and Palustriella commutata (Hedw.) Ochyra) gathered from Turkey.

作者信息

Yayintas Ozlem Tonguc, Alpaslan Duygu, Karagul Yuceer Yonca, Yilmaz Selehattin, Sahiner Nurettin

机构信息

a Department of Fisheries, Canakkale Applied Sciences , Canakkale Onsekiz Mart University , Canakkale , Turkey.

b Faculty of Engineering, Chemical Engineering Department , Yuzuncu Yil University , Van , Turkey.

出版信息

Nat Prod Res. 2017 Sep;31(18):2169-2173. doi: 10.1080/14786419.2016.1277355. Epub 2017 Jan 9.

Abstract

Cinclidotus fontinaloides (Hedw.) P. Beauv. and Palustriella commutata (Hedw.) Ochyra were used in this study. The chemical composition of mosses in the ethanol and water extract was determined using GC-MS and FT-IR. Antioxidant activities of moss extracts were tested by total phenol and ABTS methods. Trolox equivalent value (TEAC) of mosses was calculated as 26 ± 0.32 and 10 ± 0.22 mg/g. The amounts of the flavonoid compounds were calculated as mg gallic acid. Gallic acid equivalent value of mosses was calculated as 587 ± 0.55 and 496 ± 0.22 mg/g. The extracts antimicrobial activity was tested against Escherichia coli (ATCC 8739), Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231). Significant antibacterial effect was observed for C. fontinaloides and P. commutata in ethanol extract. Total anthocyanin activities of C. fontinaloides and P. commutata were determined as 50 ± 0.17 and 144 ± 0.33 mg/L.

摘要

本研究使用了溪苔(Cinclidotus fontinaloides (Hedw.) P. Beauv.)和多变泽藓(Palustriella commutata (Hedw.) Ochyra)。采用气相色谱-质谱联用(GC-MS)和傅里叶变换红外光谱(FT-IR)测定了苔藓乙醇提取物和水提取物的化学成分。通过总酚法和ABTS法测试了苔藓提取物的抗氧化活性。苔藓的Trolox当量值(TEAC)计算为26±0.32和10±0.22毫克/克。黄酮类化合物的含量以没食子酸毫克数计算。苔藓的没食子酸当量值计算为587±0.55和496±0.22毫克/克。测试了提取物对大肠杆菌(ATCC 8739)、枯草芽孢杆菌(ATCC 6633)、金黄色葡萄球菌(ATCC 6538)、铜绿假单胞菌(ATCC 10145)和白色念珠菌(ATCC 10231)的抗菌活性。在乙醇提取物中观察到溪苔和多变泽藓具有显著的抗菌效果。溪苔和多变泽藓的总花青素活性分别测定为50±0.17和144±0.33毫克/升。

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