Rocchigiani M, Pescaglini M, Sestini S, Micheli V, Ricci C
Institute of Biological Chemistry, University of Siena, Italy.
J Int Med Res. 1989 Sep-Oct;17(5):461-6. doi: 10.1177/030006058901700508.
An increase in the density of erythrocytes was observed after storage of whole blood for 30 days at 4 degrees C in either acid citrate-dextrose or citrate-phosphate-dextrose-adrenaline. Glucose-6-phosphate dehydrogenase activity in unfractionated red blood cell lysates did not vary with the storage time. Enzyme activity in the lighter fraction separated by density gradient centrifugation was higher than that in heavier fractions. The decline in glucose-6-phosphate dehydrogenase activity with density was less marked after storage of whole blood for 30 days. It is suggested that density modifications are not related to the ageing of erythrocytes and additional mechanisms may be involved.
在4℃下，将全血分别储存在酸性枸橼酸盐 - 葡萄糖或枸橼酸盐 - 磷酸盐 - 葡萄糖 - 肾上腺素中30天后，观察到红细胞密度增加。未分级的红细胞裂解物中的葡萄糖 - 6 - 磷酸脱氢酶活性不随储存时间而变化。通过密度梯度离心分离的较轻组分中的酶活性高于较重组分中的酶活性。全血储存30天后，葡萄糖 - 6 - 磷酸脱氢酶活性随密度的下降不太明显。提示密度变化与红细胞老化无关，可能涉及其他机制。
