School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
Sci Rep. 2017 Jan 9;7:40395. doi: 10.1038/srep40395.
Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.
通过仿生涂层合成了多巴胺功能化介孔洋葱状二氧化硅（DPMS），并通过物理吸附将脂肪酶 Candida sp. 99-125（LCS）固定在 DPMS 中（LCS@DPMS）。通过 SEM、TEM、BET 和 FT-IR 对 DPMS 进行了表征，结果表明 DPMS 具有清晰的多壳结构，比表面积为 419 m/g。详细研究了 LCS@DPMS 的活性、pH 稳定性、热稳定性、储存稳定性和可重复使用性。与游离脂肪酶和 LCS@MS（通过物理吸附固定在未功能化介孔洋葱状二氧化硅中的脂肪酶）相比，LCS@DPMS 的稳定性得到了显著提高。所有结果表明 DPMS 对脂肪酶固定具有高效和改善的稳定性。
