多巴胺功能化介孔洋葱状氧化硅作为固定化脂肪酶 Candida sp. 99-125 的新型基质。
Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125.
机构信息
School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
出版信息
Sci Rep. 2017 Jan 9;7:40395. doi: 10.1038/srep40395.
Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.
通过仿生涂层合成了多巴胺功能化介孔洋葱状二氧化硅（DPMS），并通过物理吸附将脂肪酶 Candida sp. 99-125（LCS）固定在 DPMS 中（LCS@DPMS）。通过 SEM、TEM、BET 和 FT-IR 对 DPMS 进行了表征，结果表明 DPMS 具有清晰的多壳结构，比表面积为 419 m/g。详细研究了 LCS@DPMS 的活性、pH 稳定性、热稳定性、储存稳定性和可重复使用性。与游离脂肪酶和 LCS@MS（通过物理吸附固定在未功能化介孔洋葱状二氧化硅中的脂肪酶）相比，LCS@DPMS 的稳定性得到了显著提高。所有结果表明 DPMS 对脂肪酶固定具有高效和改善的稳定性。
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