Szymandera-Buszka Krystyna, Hęś Marzanna, Waszkowiak Katarzyna, Jędrusek-Golińska Anna
Department of Human Nutrition and Food Technology, Poznań University of Life Sciences, Poland.
Acta Sci Pol Technol Aliment. 2014 Oct-Dec;13(4):393-401. doi: 10.17306/J.AFS.2014.4.6.
The aim of the study was to determine the effect of pasteurisation and sterilization of model systems of minced chicken meat in the presence of low or high-oxidised pork lard, soy and sunflower oil, as well as casein hydrolysate and rosemary extract, on losses of thiamine in model systems.
In the samples, the thiamine content was analysed periodically by thiochromium method, as well as rate of lipid oxidation based on measurement of peroxide value (PV) by iodometric method and p-anisidine value (AV) by spectrophotometric method.
It was observed that the thiamine losses in model systems of minced chicken after pasteurisation (61-71%) were higher than after sterilization (57-67%). Introduction of high-oxidised fat increased the total thiamine losses both during thermal processing and storage of meat samples (to 23%). A strong relationship was established between thiamine losses and rate of fat oxidation. The lowest total thiamine losses were observed in the samples with low-oxidised pork lard. Antioxidant addition (rosemary extract or casein hydrolysate) into meat samples limited the thiamine losses. However, the effect depended on oxidation of fat that was mixed with meat. In the samples with low-oxidised fat, higher protective effect was found for rosemary extract (7-11%). In the samples with high-oxidised fat, casein hydrolysate was superior to rosemary extract (14%).
In order to increase the stability of thiamine in pasteurized or sterilized meat products with fats, the influence of fat type and its oxidative stability should be taken under consideration. Moreover, the addition of rosemary extract or casein hydrolysate has impact on the thiamine losses since it slows down lipid oxidation to a significant extent.
本研究的目的是确定在存在低氧化或高氧化的猪油、大豆油和葵花籽油,以及酪蛋白水解物和迷迭香提取物的情况下,对鸡肉末模型体系进行巴氏杀菌和灭菌处理对模型体系中硫胺素损失的影响。
定期采用硫色素法分析样品中的硫胺素含量,并基于碘量法测定过氧化值(PV)和分光光度法测定对茴香胺值(AV)来分析脂质氧化速率。
观察到,鸡肉末模型体系经巴氏杀菌后的硫胺素损失(61 - 71%)高于灭菌后的损失(57 - 67%)。引入高氧化脂肪会增加肉类样品热处理和储存期间硫胺素的总损失(高达23%)。硫胺素损失与脂肪氧化速率之间建立了密切关系。在含有低氧化猪油的样品中观察到硫胺素的总损失最低。向肉类样品中添加抗氧化剂(迷迭香提取物或酪蛋白水解物)可限制硫胺素损失。然而,效果取决于与肉混合的脂肪的氧化情况。在低氧化脂肪的样品中,迷迭香提取物的保护效果更佳(7 - 11%)。在高氧化脂肪的样品中,酪蛋白水解物优于迷迭香提取物(14%)。
为提高含脂肪的巴氏杀菌或灭菌肉制品中硫胺素的稳定性,应考虑脂肪类型及其氧化稳定性的影响。此外,添加迷迭香提取物或酪蛋白水解物对硫胺素损失有影响,因为它能在很大程度上减缓脂质氧化。