Girona Neuroimmunology and Multiple Sclerosis Unit (UNIEM), Dr. Josep Trueta University Hospital and Girona Biomedical Research Institute (IDIBGI), Girona, Spain/Red Española de Esclerosis Múltiple (REEM), Madrid, Spain.
Department of Diabetes, Endocrinology and Nutrition, Girona Biomedical Research Institute (IDIBGI), Girona, Spain.
Mult Scler. 2017 Nov;23(13):1716-1726. doi: 10.1177/1352458516684213. Epub 2017 Jan 9.
MicroRNAs (miRNAs) are non-coding RNAs that regulate cellular processes by controlling protein translation and mRNA degradation.
We aimed to explore the miRNA signature of multiple sclerosis (MS) patients versus controls and the possibility that patients with lipid-specific oligolconal IgM bands (LS_OCMB), a predictor of a more severe disease course, may have a distinct profile.
An extensive profile of 754 miRNAs was evaluated in the cerebrospinal fluid (CSF) of 14 women using TaqMan low-density arrays. Differentially expressed miRNAs together with others previously identified in the literature were validated in an extended sample of 86 MS patients (39 LS_OCMB+) and 55 controls.
We detected higher levels of miR-150 in MS patients and especially in those with LS_OCMB+. Other miRNAs (miR-328, miR-30a-5p and miR-645) were up-regulated in MS patients compared to controls while miR-21, miR-199a-3p, miR-191, miR-365, miR-106a and miR-146a showed down-regulated expression. Considering only patients with LS_OCMB+, we also detected up-regulation of miR-30a-5p, miR-150 and miR-645 and down-regulation of miR-191 compared to controls.
Our study confirms the recent findings regarding the deregulated expression of miR-150 not only with MS but also with the presence of LS_OCMB. This study highlights the potential utility of miRNAs in CSF as biomarkers for MS.
MicroRNAs（miRNAs）是通过控制蛋白质翻译和 mRNA 降解来调节细胞过程的非编码 RNA。
我们旨在探索多发性硬化症（MS）患者与对照之间的 miRNA 特征，以及脂质特异性寡克隆 IgM 带（LS_OCMB）患者（预示疾病更严重）是否可能具有独特的特征。
使用 TaqMan 低密度阵列评估了 14 名女性脑脊液（CSF）中 754 种 miRNA 的广泛特征。在 86 名 MS 患者（39 名 LS_OCMB+）和 55 名对照的扩展样本中验证了差异表达的 miRNA 以及文献中先前鉴定的其他 miRNA。
我们检测到 MS 患者，尤其是具有 LS_OCMB+的患者中 miR-150 的水平更高。与对照组相比，其他 miRNA（miR-328、miR-30a-5p 和 miR-645）在 MS 患者中上调，而 miR-21、miR-199a-3p、miR-191、miR-365、miR-106a 和 miR-146a 的表达下调。仅考虑具有 LS_OCMB+的患者，我们还检测到 miR-30a-5p、miR-150 和 miR-645 的上调，以及 miR-191 的下调与对照组相比。
我们的研究证实了最近关于 miR-150 不仅在 MS 中而且在 LS_OCMB 存在时表达失调的发现。这项研究强调了 CSF 中 miRNA 作为 MS 生物标志物的潜在效用。
