用于 mRNA 和 MRI 造影剂递送的双功能类脂纳米颗粒。
Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents.
机构信息
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA.
Small Animal Imaging Center, The Ohio State University, Columbus, Ohio 43210, USA.
出版信息
Nanoscale. 2017 Jan 26;9(4):1575-1579. doi: 10.1039/c6nr08496f.
Abstract
Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparable or a slightly higher delivery efficiency of mRNA compared to the initial TT3 LLNs. Moreover, a strong MRI signal was observed in the cell pellets treated with TT3-Gd18 LLNs. More importantly, TT3-Gd18 LLNs demonstrated an efficient delivery of mRNA and Gd contrast agents in vivo.
摘要
多功能纳米材料具有独特的性质，除其他外，还能促进治疗和诊断应用。在此，我们开发了双重功能的类脂质体纳米粒子，用于同时递 mRNA 和磁共振成像 (MRI) 造影剂，以表达功能性蛋白质并提供实时可视化。TT3-Gd18 LLNs 被确定为一种主导配方，它能够封装 91%的 mRNA 和 74%的 Gd。与初始 TT3 LLNs 相比，该配方显示出相当或略高的 mRNA 递药效率。此外，在用 TT3-Gd18 LLNs 处理的细胞沉淀中观察到强烈的 MRI 信号。更重要的是，TT3-Gd18 LLNs 在体内有效地递 mRNA 和 Gd 造影剂。
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