Li Bin, Dong Yizhou
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, 496 W. 12th Ave., Columbus, OH, 43210, USA.
Methods Mol Biol. 2017;1632:207-217. doi: 10.1007/978-1-4939-7138-1_13.
Lipid-like nanoparticles (LLNs) have shown great promise for nucleic acid delivery. Recently, we have developed N ,N ,N -tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (TT) derived lipid-like compounds, formulated them into TT LLNs for mRNA delivery, and applied an orthogonal array design to facilitate formulation optimization. This chapter focuses on the following contents relevant to lipid-like nanoparticles: formulation method, particle characterization, orthogonal array design, and in vitro assays.
类脂纳米颗粒（LLNs）在核酸递送方面已显示出巨大的潜力。最近，我们开发了由N，N，N-三（2-氨基乙基）苯-1,3,5-三羧酰胺（TT）衍生的类脂化合物，将其制备成用于mRNA递送的TT LLNs，并应用正交阵列设计来促进制剂优化。本章重点介绍与类脂纳米颗粒相关的以下内容：制剂方法、颗粒表征、正交阵列设计和体外测定。
