用于mRNA递送的类脂质纳米颗粒的制备与优化
Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery.
作者信息
Li Bin, Dong Yizhou
机构信息
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, 496 W. 12th Ave., Columbus, OH, 43210, USA.
出版信息
Methods Mol Biol. 2017;1632:207-217. doi: 10.1007/978-1-4939-7138-1_13.
Abstract
Lipid-like nanoparticles (LLNs) have shown great promise for nucleic acid delivery. Recently, we have developed N ,N ,N -tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (TT) derived lipid-like compounds, formulated them into TT LLNs for mRNA delivery, and applied an orthogonal array design to facilitate formulation optimization. This chapter focuses on the following contents relevant to lipid-like nanoparticles: formulation method, particle characterization, orthogonal array design, and in vitro assays.
摘要
类脂纳米颗粒（LLNs）在核酸递送方面已显示出巨大的潜力。最近，我们开发了由N，N，N-三（2-氨基乙基）苯-1,3,5-三羧酰胺（TT）衍生的类脂化合物，将其制备成用于mRNA递送的TT LLNs，并应用正交阵列设计来促进制剂优化。本章重点介绍与类脂纳米颗粒相关的以下内容：制剂方法、颗粒表征、正交阵列设计和体外测定。
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