Pacca Carolina Colombelli, Marques Rafael Elias, Espindola José Wanderlan P, Filho Gevânio B O Oliveira, Leite Ana Cristina Lima, Teixeira Mauro Martins, Nogueira Mauricio L
Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitarias, Faculdade de Medicina de São José do Rio Preto - FAMERP, 15090-000, São José do Rio Preto, SP, Brazil; Faceres Medical School, 15090-305, São José do Rio Preto, SP, Brazil.
Laboratório de Imunofarmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.
Biomed Pharmacother. 2017 Mar;87:381-387. doi: 10.1016/j.biopha.2016.12.112. Epub 2017 Jan 6.
Arboviruses, arthropod-borneviruses, are frequency associated to human outbreak and represent a serious health problem. The genus Flavivirus, such as Yellow Fever Virus (YFV) and Saint Louis Encephalitis Virus (SLEV), are important pathogens with high morbidity and mortality worldwide. In Brazil, YFV is maintained in sylvatic cycle, but many cases are notified annually, despite the efficiency of vaccine. SLEV causes an acute encephalitis and is widely distributed in the Americas. There is no specific antiviral drugs for these viruses, only supporting treatment that can alleviate symptoms and prevent complications. Here, we evaluated the potential anti-YFV and SLEV activity of a series of thiosemicarbazones and phthalyl-thiazoles. Plaque reduction assay, flow cytometry, immunofluorescence and cellular viability were used to test the compounds in vitro. Treated cells showed efficient inhibition of the viral replication at concentrations that presented minimal toxicity to cells. The assays showed that phthalyl-thiazole and phenoxymethyl-thiosemicarbazone reduced 60% of YFV replication and 75% of SLEV replication.
虫媒病毒，即节肢动物传播的病毒，常常与人类疫情爆发相关，是一个严重的健康问题。黄病毒属，如黄热病病毒（YFV）和圣路易斯脑炎病毒（SLEV），是全球范围内发病率和死亡率都很高的重要病原体。在巴西，黄热病病毒在丛林循环中存在，但尽管有疫苗，每年仍有许多病例被报告。圣路易斯脑炎病毒会引发急性脑炎，在美洲广泛分布。针对这些病毒没有特效抗病毒药物，只有支持性治疗来缓解症状和预防并发症。在此，我们评估了一系列硫代氨基脲和邻苯二甲酰噻唑对黄热病病毒和圣路易斯脑炎病毒的潜在抗病毒活性。采用蚀斑减少试验、流式细胞术、免疫荧光和细胞活力检测来在体外测试这些化合物。经处理的细胞在对细胞毒性极小的浓度下显示出对病毒复制的有效抑制。试验表明，邻苯二甲酰噻唑和苯氧甲基硫代氨基脲分别使黄热病病毒复制减少60%，使圣路易斯脑炎病毒复制减少75%。
