Thamsermsang Onusa, Akarasereenont Pravit, Laohapand Tawee, Panich Uraiwan
Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
BMC Complement Altern Med. 2017 Jan 10;17(1):32. doi: 10.1186/s12906-016-1515-0.
Pain is the main symptom of most musculoskeletal disorders and can be caused by inflammation in association with oxidative stress. Thai herbal Sahatsatara formula (STF), a polyherbal formula, has been traditionally used for relieving muscle pain and limb numbness. This study aimed to investigate biologically active compounds of STF and its pharmacological effects related to antioxidant and anti-inflammatory activities.
The identification of possibly active compounds of STF was performed by high performance liquid chromatography (HPLC). Moreover, this study also assessed the free radical scavenging activities of STF and its components using DPPH radical scavenging assay and their inhibitory effects on IL-1β-induced intracellular reactive oxygen species (ROS) formation in primary human dermal fibroblasts (NHDFs) using DCFDA-flow cytometry analysis. Modulation of human gene expression by STF and its active compounds was investigated by microarray analyzed through Gene Ontology (GO) classification and pathway enrichment analysis.
HPLC analysis has revealed the presence of gallic acid (GA) and piperine (PP) as the major compounds in STF extracts. Our finding discovered that STF and its active compounds (GA and PP) yielded free radical scavenging activities and abilities to inhibit IL-1β-induced cellular ROS formation in NHDFs. Furthermore, microarray analysis demonstrated that a total of 84 genes (54 upregulated and 30 downregulated) were significantly affected by IL-1β involved in inflammatory cytokines, chemokines, transcription factors, cell adhesion molecules and other immunomodulators participating in NF-κB signaling. The significantly upregulated genes in IL-1β-treated in NHDFs participate in interleukin and cholecystokinin (CCRK) signaling pathways. The GO analysis of the target genes showed that all test compounds including indomethacin, STF and its active compounds, can downregulate the genes involved in NF-кB signaling pathway in IL-1β-treated NHDFs compared to the cells treated with IL-1β alone.
STF and its active compounds possessing antioxidant actions can modulate the effects of IL-1β-mediated alteration of gene expression profiles associated with inflammatory signaling in NHDFs.
疼痛是大多数肌肉骨骼疾病的主要症状，可能由炎症与氧化应激共同引起。泰国草药萨哈察塔拉配方（STF）是一种多草药配方，传统上用于缓解肌肉疼痛和肢体麻木。本研究旨在研究STF的生物活性成分及其与抗氧化和抗炎活性相关的药理作用。
通过高效液相色谱法（HPLC）对STF可能的活性成分进行鉴定。此外，本研究还使用DPPH自由基清除试验评估了STF及其成分的自由基清除活性，并使用DCFDA流式细胞术分析评估了它们对白细胞介素-1β（IL-1β）诱导的原代人皮肤成纤维细胞（NHDFs）内活性氧（ROS）形成的抑制作用。通过基因本体论（GO）分类和通路富集分析的微阵列分析研究了STF及其活性成分对人类基因表达的调节作用。
HPLC分析显示没食子酸（GA）和胡椒碱（PP）是STF提取物中的主要成分。我们的研究发现，STF及其活性成分（GA和PP）具有自由基清除活性以及抑制NHDFs中IL-1β诱导的细胞ROS形成的能力。此外，微阵列分析表明，共有84个基因（54个上调和30个下调）受到IL-1β的显著影响，这些基因涉及参与NF-κB信号传导的炎性细胞因子、趋化因子、转录因子、细胞粘附分子和其他免疫调节剂。在NHDFs中，IL-1β处理后显著上调的基因参与白细胞介素和胆囊收缩素（CCRK）信号通路。对靶基因的GO分析表明，与单独用IL-1β处理的细胞相比，所有测试化合物（包括吲哚美辛、STF及其活性成分）均可下调IL-1β处理的NHDFs中参与NF-κB信号通路的基因。
具有抗氧化作用的STF及其活性成分可调节IL-1β介导的与NHDFs中炎症信号相关的基因表达谱变化的影响。
