Kakatum Narin, Jaiarree Nuanjan, Makchucit Sunita, Itharat Arunporn
Graduate School, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
J Med Assoc Thai. 2012 Jan;95 Suppl 1:S120-6.
Twenty one plants as ingredients of Thai traditional preparation called Sahasthara for relieve muscles pain and distal numbness were studied for their antioxidant and anti-inflammatory activities.
The extracts were obtained by maceration with 95% ethanol. They were tested for their antioxidant activity by DPPH scavenging assay and anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines using Griess reagent.
The ethanolic extract of Terminalia chebula Retz. (Gall) showed the highest antioxidant activity (EC50 = 3.34 microg/ml). Its fruit extract also exhibited the most potent inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cells, followed by Atractylodes lancea and Baliospermum montanum (IC50 = 3.3, 9.7 and 12.6 microg/ ml, respectively).
The results obtained for anti-inflammatory and antioxidant activities of these plants correspond with their traditional use for muscle pain and inflammatory-related diseases.
研究21种作为泰国传统制剂“萨哈斯塔拉”（Sahasthara）成分的植物，该制剂用于缓解肌肉疼痛和远端麻木，对其抗氧化和抗炎活性进行研究。
采用95%乙醇浸渍法获得提取物。通过DPPH清除试验检测其抗氧化活性，使用格里斯试剂通过测定对脂多糖（LPS）诱导的RAW 264.7细胞系中一氧化氮（NO）产生的抑制活性来检测抗炎活性。
诃子（诃子）乙醇提取物显示出最高的抗氧化活性（EC50 = 3.34微克/毫升）。其果实提取物对RAW 264.7细胞中脂多糖（LPS）诱导的一氧化氮（NO）产生也表现出最强的抑制活性，其次是苍术和巴豆（IC50分别为3.3、9.7和12.6微克/毫升）。
这些植物的抗炎和抗氧化活性结果与其用于肌肉疼痛和炎症相关疾病的传统用途相符。
