核RNA结合蛋白RBP33影响布氏锥虫中的mRNA和剪接前导RNA丰度。
The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei.
作者信息
Cirovic Olivera, Trikin Roman, Hoffmann Anneliese, Doiron Nicholas, Jakob Martin, Ochsenreiter Torsten
机构信息
Institute of Cell Biology, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences at the University of Bern, Switzerland.
Institute of Cell Biology, University of Bern, Switzerland.
出版信息
Mol Biochem Parasitol. 2017 Mar;212:16-20. doi: 10.1016/j.molbiopara.2016.12.008. Epub 2017 Jan 6.
RNA recognition motif (RRM) containing proteins are important regulators of gene expression in trypanosomes. Here we expand our current knowledge on the exclusively nuclear localized RRM domain containing protein RBP33 of Trypanosoma brucei. Overexpression of RBP33 leads to a quick growth arrest in G2/M in bloodstream form cells likely due to an overall mRNA- and spliced leader abundance decrease while the ribosomal RNAs remain unaffected. The recombinant RBP33 binds to poly(A) and random sequence RNA in vitro confirming its role as a RNA binding protein. Finally super-resolution microscopy detects RBP33 in small punctae throughout the nucleus and surrounding the nucleolus, however the signal is depleted inside the nucleolus.
含RNA识别基序（RRM）的蛋白质是锥虫基因表达的重要调节因子。在此，我们拓展了对布氏锥虫仅定位于细胞核的含RRM结构域蛋白RBP33的现有认识。RBP33的过表达导致血流形式细胞在G2/M期快速生长停滞，这可能是由于整体mRNA和剪接前导序列丰度降低，而核糖体RNA不受影响。重组RBP33在体外与聚腺苷酸和随机序列RNA结合，证实了其作为RNA结合蛋白的作用。最后，超分辨率显微镜在整个细胞核和核仁周围的小点中检测到RBP33，然而核仁内部信号缺失。
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